Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer
Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer
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DOI：
10.1016/j.bbrc.2013.05.101
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发表时间：
2013-06-28
影响因子：
3.1
通讯作者：
Satoh, Kennichi
中科院分区：
文献类型：
--
作者：
Nakagawa, Takayuki;Endo, Hiroyuki;Satoh, Kennichi
HOTAIR是长链非编码RNA之一，其表达与多种癌症的预后和转移相关。我们发现 HOTAIR 表达在非小细胞肺癌（NSCLC）的发展中具有重要作用。在本研究中，我们通过定量实时 RT-PCR 检测了 77 例 NSCLC、其相应的正常肺组织和 6 例脑转移瘤中 HOTAIR 的表达。 HOTAIR高表达（肿瘤/正常比≥2）在17例（22.1%）患者中检测到，多见于晚期、淋巴结转移或淋巴管侵犯、无病间隔短的患者。此外，与原发癌组织相比，脑转移瘤显示出显着更高的 HOTAIR 表达。表达 HOTAIR 的 A549 细胞在体外表现出诱导的细胞迁移和不依赖贴壁的细胞生长。这些结果表明HOTAIR的表达增强了NSCLC细胞的侵袭行为。 (C) 2013 Elsevier Inc. 保留所有权利。
HOTAIR is one of long non-coding RNAs and its expression correlates with the prognosis and metastasis in various cancers. We showed that HOTAIR expression has an important role in the development of non-small cell lung cancer (NSCLC). In this study, we examined the expression of HOTAIR in 77 NSCLCs, their corresponding normal lung tissues and 6 brain metastases by quantitative real-time RT-PCR. High expression of HOTAIR (tumor/normal ratio >= 2) was detected in 17 patients (22.1%) and was frequently found in patients with advanced stage, lymph node metastasis or lymph-vascular invasion and short disease free interval. Furthermore, brain metastases show significantly higher HOTAIR expression compared to primary cancer tissues. HOTAIR-expressing A549 cells showed induced cell migration and anchorage-independent cell growth in vitro. These results indicate the expression of HOTAIR enhanced the aggressive behavior of NSCLC cells. (C) 2013 Elsevier Inc. All rights reserved.