Prevention of HIV-2 and SIVsm infection by passive immunization in cynomolgus monkeys
Prevention of HIV-2 and SIVsm infection by passive immunization in cynomolgus monkeys
复制标题
食蟹猴被动免疫预防 HIV-2 和 SIVsm 感染
作者：
P. Putkonen;R. Thorstensson;L. Ghavamzadeh;J. Albert;K. Hild;G. Biberfeld;E. Norrby
猕猴感染猴免疫缺陷病毒（SIV）1，2和人类免疫缺陷病毒2型（HIV-2）3，4是研究HIV免疫治疗和疫苗接种以及测试抗病毒药物的有用模型。疫苗研究表明，免疫猴具有保护性免疫力4 -10，这表明有可能开发出预防人类HIV感染的疫苗，尽管仍存在许多障碍。如果能够阐明保护性免疫的基础，将有助于艾滋病毒疫苗的设计。被动免疫预防提供了一种方法来确定抗体在保护免受感染中的相对作用。我们已经研究了抗体的转移是否可以预防食蟹猴中的HIV-2和SIVsm（来源于白眉猴的SIV）感染。收集具有高抗体滴度的血清，进行热处理，并在用10-100个猴感染剂量的活同源病毒攻击前6小时注射到幼稚动物中。用正常猴血清（n = 6）或无血清（n = 39）处理的所有对照动物均被攻毒病毒感染，而用9 ml kg−1剂量的含抗体血清预处理的7只动物中有5只抵抗感染。因此，被动转移的抗体可以保护非人灵长类动物免受低剂量慢病毒攻击。
INFECTION of macaques with simian immunodeficiency virus (SIV)1,2 and human immunodeficiency virus type 2 (HIV-2) 3,4 are useful models for studies of immunotherapy and vaccination against HIV as well as for testing of antiviral drugs. Vaccine research showing protective immunity in immunized monkeys4–10 has indicated that it will be possible to develop a vaccine for prevention of human HIV infection, although many hurdles remain. The design of an HIV vaccine would be helped if the basis of the protective immunity could be elucidated. Passive immune prophylaxis offers a means to determine the relative role of antibodies in protection against infection. We have studied whether a transfer of antibodies can prevent HIV-2 and SIVsm (SIV of sooty mangabey origin) infection in cynomolgus monkeys. Sera with high antibody titres were collected, heat-treated and injected into naive animals 6 h before challenge with 10–100 monkey-infectious doses of live homologous virus. All control animals treated with normal monkey serum (n = 6) or no serum (n = 39) became infected by the challenge virus, whereas five out of seven animals pretreated with antibody-containing serum at a dose of 9 ml kg−1 resisted infection. Thus passively transferred antibodies can protect against a low-dose lentivirus challenge in a nonhuman primate.
人类免疫缺陷病毒（HIV）免疫球蛋白未能保护黑猩猩免受艾滋病毒的实验挑战。
DOI：
10.1073/pnas.85.18.6944
发表时间：
1988
影响因子：
11.1
作者：
Prince,AM;Horowitz,B;Baker,L;Shulman,RW;Ralph,H;Valinsky,J;Cundell,A;Brotman,B;Boehle,W;Rey,F
通讯作者：
Rey,F
恒河猴免受猿猴免疫缺陷病毒感染的疫苗保护。
影响因子：
1.5
作者：
Carlson,JR;McGraw,TP;Keddie,E;Yee,JL;Rosenthal,A;Langlois,AJ;Dickover,R;Donovan,R;Luciw,PA;Jennings,MB
通讯作者：
Jennings,MB