Structuring of bacterioplankton communities by specific dissolved organic carbon compounds
Structuring of bacterioplankton communities by specific dissolved organic carbon compounds
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DOI：
10.1111/j.1462-2920.2012.02804.x
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发表时间：
2012-09-01
影响因子：
5.1
通讯作者：
Pinhassi, Jarone
中科院分区：
文献类型：
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作者：
Gomez-Consarnau, Laura;Lindh, Markus V.;Pinhassi, Jarone
微生物在海洋中大量溶解有机碳库循环中的主要作用已得到充分证实。然而，目前尚不清楚是否特定的细菌优先利用特定的碳化合物，以及这些化合物是否具有塑造细菌群落组成的潜力。在地中海、波罗的海和北海（Skagerrak）进行的富集实验表明，在大多数情况下，对海洋细菌生长具有重要作用的不同低分子量有机化合物（如氨基酸、葡萄糖、二甲基磺胺丙酸酯、醋酸酯或丙酮酸酯）对细菌生长的刺激是不同的。变性梯度凝胶电泳指纹图谱和16S rRNA基因测序显示，一些细菌种类丰富，对特定碳化合物的富集具有高度特异性（如醋酸不动杆菌sp. B1-A3或冻干单胞菌sp. B3-U1与葡萄糖）。相比之下，其他种型的相对丰度随着几种或全部所研究的碳化合物的富集而增加（例如Neptuniibacter sp. M2-A4与醋酸酯、丙酮酸酯和二甲基磺酰丙酸酯，Thalassobacter sp. M3-A3与丙酮酸酯和氨基酸）。此外，在几个实验中，不同的碳化合物引发了优势种型的独特组合的发展。这些结果表明，细菌在利用特定碳化合物的能力上存在很大差异，一些细菌是专家，而另一些细菌则有更广泛的策略。因此，溶解有机碳库的供应或组成的变化可以作为构建浮游细菌群落的选择性力量。
The main role of microorganisms in the cycling of the bulk dissolved organic carbon pool in the ocean is well established. Nevertheless, it remains unclear if particular bacteria preferentially utilize specific carbon compounds and whether such compounds have the potential to shape bacterial community composition. Enrichment experiments in the Mediterranean Sea, Baltic Sea and the North Sea (Skagerrak) showed that different low-molecular-weight organic compounds, with a proven importance for the growth of marine bacteria (e.g. amino acids, glucose, dimethylsulphoniopropionate, acetate or pyruvate), in most cases differentially stimulated bacterial growth. Denaturing gradient gel electrophoresis fingerprints and 16S rRNA gene sequencing revealed that some bacterial phylotypes that became abundant were highly specific to enrichment with specific carbon compounds (e.g. Acinetobacter sp. B1-A3 with acetate or Psychromonas sp. B3-U1 with glucose). In contrast, other phylotypes increased in relative abundance in response to enrichment with several, or all, of the investigated carbon compounds (e.g. Neptuniibacter sp. M2-A4 with acetate, pyruvate and dimethylsulphoniopropionate, and Thalassobacter sp. M3-A3 with pyruvate and amino acids). Furthermore, different carbon compounds triggered the development of unique combinations of dominant phylotypes in several of the experiments. These results suggest that bacteria differ substantially in their abilities to utilize specific carbon compounds, with some bacteria being specialists and others having a more generalist strategy. Thus, changes in the supply or composition of the dissolved organic carbon pool can act as selective forces structuring bacterioplankton communities.