Ontogeny of synaptic phosphoproteins in brain.
Ontogeny of synaptic phosphoproteins in brain.
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大脑中突触磷蛋白的个体发育。
DOI：
10.1073/pnas.75.8.4037
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发表时间：
1978
影响因子：
11.1
通讯作者：
P. Greengard
中科院分区：
文献类型：
--
作者：
S. Lohmann;T. Ueda;P. Greengard
在大鼠和豚鼠大脑中，研究了两种内源性cAMP依赖蛋白激酶(ATP：蛋白磷酸转移酶；EC 2.7.1.37)的个体发育。这些内源性底物，被称为蛋白质Ia和Ib，在其他研究中已被证明是神经组织特有的，并在成人大脑的突触膜和突触小泡部分富含。在本研究中，大鼠大脑和豚鼠大脑中的蛋白Ia和Ib在主要突触发生期间显著增加，其中突触的形态发育分别为出生后和出生前。无论是通过测量突触膜部分蛋白Ia和Ib的内源性磷酸化，还是通过测量提取的蛋白Ia和Ib在添加蛋白激酶的情况下的磷酸化，都得到了类似的结果。
The ontogeny of two endogenous substrates for cyclic AMP-dependent protein kinase (ATP:protein phosphotransferase; EC 2.7.1.37) has been studied in rat and guinea pig cerebrum. These endogenous substrates, referred to as proteins Ia and Ib, have been shown in other studies to be specific to nervous tissue and to be enriched in the synaptic membrane and synaptic vesicle fractions of adult brain. In the present study, proteins Ia and Ib were shown to increase markedly during the time of major synaptogenesis in rat cerebrum and guinea pig cerebrum, in which the morphological development of synapses is predominantly postnatal and prenatal, respectively. Similar results were obtained either by measuring endogenous phosphorylation of proteins Ia and Ib in the synaptic membrane fraction or by measuring phosphorylation of extracted proteins Ia and Ib with added protein kinase.