Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysis via suppression of sirtuin 3 in pancreatic cancer.
Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysis via suppression of sirtuin 3 in pancreatic cancer.
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锌指 E 盒结合同源盒 1 通过抑制胰腺癌中的 Sirtuin 3 介导有氧糖酵解
DOI：
10.3748/wjg.v24.i43.4893
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发表时间：
2018-11-21
影响因子：
4.3
通讯作者：
Yu XJ
中科院分区：
文献类型：
--
作者：
Xu WY;Hu QS;Qin Y;Zhang B;Liu WS;Ni QX;Xu J;Yu XJ
AIM To uncover the roles of tumor-promoting gene ZEB1 in aerobic glycolysis regulation and shed light on the underlying molecular mechanism. METHODS Endogenous zinc finger E-box binding homeobox-1 (ZEB1) was silenced using a lentivirus-mediated method, and the impact of ZEB1 and methyl-CpG binding domain protein 1 (MBD1) on aerobic glycolysis was measured using seahorse cellular flux analyzers, reactive oxygen species quantification, and mitochondrial membrane potential measurement. The interaction between ZEB1 and MBD1 was assessed by co-immunoprecipitation and immunofluorescence assays. The impact of ZEB1 and MBD1 interaction on sirtuin 3 (SIRT3) expression was confirmed by quantitative polymerase chain reaction, western blotting, and dual-luciferase and chromatin-immunoprecipitation assays. RESULTS ZEB1 was a positive regulator of aerobic glycolysis in pancreatic cancer. ZEB1 transcriptionally silenced expression of SIRT3, a mitochondrial-localized tumor suppressor, through interaction with MBD1. CONCLUSION ZEB1 silenced SIRT3 expression via interaction with MBD1 to promote aerobic glycolysis in pancreatic cancer.
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影响因子：
9
作者：
Passacantilli I;Panzeri V;Bielli P;Farini D;Pilozzi E;Fave GD;Capurso G;Sette C
通讯作者：
Sette C
影响因子：
3.8
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通讯作者：
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影响因子：
64.5
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通讯作者：
Wei W
影响因子：
50.3
作者：
Finley LW;Carracedo A;Lee J;Souza A;Egia A;Zhang J;Teruya-Feldstein J;Moreira PI;Cardoso SM;Clish CB;Pandolfi PP;Haigis MC
通讯作者：
Haigis MC
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影响因子：
11.5
作者：
Ji, Shunrong;Qin, Yi;Yu, Xianjun
通讯作者：
Yu, Xianjun