PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth in Arabidopsis thaliana
PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth in Arabidopsis thaliana
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DOI：
10.1073/pnas.1814015116
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发表时间：
2019-02-26
影响因子：
11.1
通讯作者：
Kleine-Vehn, Juergen
中科院分区：
文献类型：
--
作者：
Feraru, Elena;Feraru, Mugurel I.;Kleine-Vehn, Juergen
温度调节植物整个生命周期的生长和发育。高温(HT)触发气生组织生长素生物合成依赖性生长。另一方面，生长素在羟色胺诱导的根生长中的作用目前还存在争议。在这里，我们证明了假定的细胞内生长素载体PIN样蛋白6(PILS6)是器官生长的负调控因子，其丰度对羟色胺高度敏感。PILS6定位于内质网，限制了生长素的核可获得性，从而减少了生长素信号输出。羟色胺抑制PILS6蛋白的丰度，从而影响根中依赖PILS6的生长素信号转导和根的伸长。因此，我们假设PILS6是将HT与根中的生长素反应联系起来的另一种机制的一部分。
Temperature modulates growth and development throughout the entire lifecycle of a plant. High temperature (HT) triggers the auxin biosynthesis-dependent growth in aerial tissues. On the other hand, the contribution of auxin to HT-induced root growth is currently under debate. Here we show that the putative intracellular auxin carrier PIN-LIKES 6 (PILS6) is a negative regulator of organ growth and that its abundance is highly sensitive to HT. PILS6 localizes to the endoplasmic reticulum and limits the nuclear availability of auxin, consequently reducing the auxin signaling output. HT represses the PILS6 protein abundance, which impacts on PILS6-dependent auxin signaling in roots and root expansion. Accordingly, we hypothesize that PILS6 is part of an alternative mechanism linking HT to auxin responses in roots.