Genome-wide survey for genes horizontally transferred from cellular organisms to baculoviruses
Genome-wide survey for genes horizontally transferred from cellular organisms to baculoviruses
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DOI：
10.1093/molbev/msg107
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发表时间：
2003-06-01
影响因子：
10.7
通讯作者：
Friedman, R
中科院分区：
文献类型：
--
作者：
Hughes, AL;Friedman, R
以杆状病毒科颗粒病毒属和核多角体病毒属的22个保守蛋白家族为基础，重建了13个病毒种的系统发育，并对这些病毒的全基因组进行了全面的同源性搜索和系统发育分析，以检测细胞生物的水平基因转移。在6个蛋白家族（DNA连接酶、核糖核苷酸还原酶1、SNF2全局反激活因子、凋亡抑制剂、几丁质酶和udp -葡萄糖基转移酶）中发现了具有统计学意义的水平转移证据。已知其中三个科在这些病毒感染昆虫宿主中起关键作用。有证据表明，其中两种（细胞凋亡抑制剂和udp -葡萄糖基转移酶）来自昆虫宿主。相比之下，这些病毒中编码几丁质酶的基因显然来自一组细菌（变形细菌的γ分支），它们利用几丁质酶分解真菌几丁质。
The phylogeny of 13 viral species in the genera Granulovirus and Nucleopolyhedrovirus (family Baculoviridae) was reconstructed on the basis of 22 conserved protein families shared by all species, and a comprehensive homology search and phylogenetic analysis of the complete genomes of these viruses was used to test for horizontal gene transfer from cellular organisms. Statistically significant evidence of horizontal transfer was found in the case of six protein families (DNA ligase, ribonucleotide reductase 1, SNF2 global transactivator, inhibitor of apoptosis, chitinase, and UDP-glucosyltransferase). Three of these families are known to play key roles in the infection of insect hosts by these viruses. There was evidence that two of these (inhibitor of apoptosis and UDP-glucosyltransferase) were derived from the insect host. By contrast, the gene encoding chitinase in these viruses was evidently derived from a group of bacteria (the gamma subdivision of proteobacteria), which use chitinase to break down fungal chitins.