Engineering an in vitro model of a functional ligament from bone to bone.
Engineering an in vitro model of a functional ligament from bone to bone.
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DOI：
10.1089/ten.tea.2010.0039
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发表时间：
2010-08
影响因子：
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通讯作者：
J. Paxton;L. Grover;K. Baar
中科院分区：
文献类型：
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作者：
J. Paxton;L. Grover;K. Baar
对于在运动过程中传递载荷的肌肉骨骼组织，组织之间的界面对于最小化损伤至关重要。因此，在工程化肌肉骨骼组织内复制功能界面对于将该技术成功转移到临床至关重要。这项工作的目标是在体外快速设计韧带等同物，其中包含软组织肌腱和硬组织骨模拟物。使用铸造透钙磷石（CaHPO（4）·2 H（2）O）锚钉模拟骨和包埋成纤维细胞的纤维蛋白凝胶形成肌腱，实现了这一目标。构建体在7天内形成。接种后14天，透钙磷石与肌腱的界面能承受9.51 ± 1.7kPa的应力，肌腱的杨氏模量达到0.16 ± 0.03MPa。用抗坏血酸和脯氨酸处理后，肌腱的胶原含量增加（从1.34% ± 0.2%增加到8.34% ± 0.37%），界面强度增加（29.24 ± 6 kPa），弹性模量增加（2.69 ± 0.25 MPa）。加入转化生长因子-β后，胶原含量进一步增加（11.25% ± 0.39%），界面强度进一步增加（42 ± 8 kPa），肌腱弹性模量进一步增加（5.46 ± 0.68 MPa）。扫描电子显微镜和拉曼显微镜都表明透钙磷石和肌腱之间的界面模仿了在附着点的体内潮标。这项工作描述了一个重要的一步，发展组织工程韧带修复韧带断裂在人类。
For musculoskeletal tissues that transmit loads during movement, the interfaces between tissues are essential to minimizing injury. Therefore, the reproduction of functional interfaces within engineered musculoskeletal tissues is critical to the successful transfer of the technology to the clinic. The goal of this work was to rapidly engineer ligament equivalents in vitro that contained both the soft tissue sinew and a hard tissue bone mimetic. This goal was achieved using cast brushite (CaHPO(4)·2H(2)O) anchors to mimic bone and a fibrin gel embedded with fibroblasts to create the sinew. The constructs formed within 7 days. Fourteen days after seeding, the interface between the brushite and sinew could withstand a stress of 9.51 ± 1.7 kPa before failure and the sinew reached a Young's modulus value of 0.16 ± 0.03 MPa. Treatment with ascorbic acid and proline increased the collagen content of the sinew (from 1.34% ± 0.2% to 8.34% ± 0.37%), strength of the interface (29.24 ± 6 kPa), and modulus of the sinew (2.69 ± 0.25 MPa). Adding transforming growth factor-β resulted in a further increase in collagen (11.25% ± 0.39%), interface strength (42 ± 8 kPa), and sinew modulus (5.46 ± 0.68 MPa). Both scanning electron and Raman microscopy suggested that the interface between the brushite and sinew mimics the in vivo tidemark at the enthesis. This work describes a major step toward the development of tissue-engineered ligaments for the repair of ligament ruptures in humans.