Reference values for urinary steroids in Japanese newborn infants: Gas chromatography/mass spectrometry in selected ion monitoring
Reference values for urinary steroids in Japanese newborn infants: Gas chromatography/mass spectrometry in selected ion monitoring
复制标题
DOI：
10.1507/endocrj.50.783
复制
发表时间：
2003-12-01
影响因子：
2
通讯作者：
Matsuo, N
中科院分区：
文献类型：
--
作者：
Homma, K;Hasegawa, T;Matsuo, N
使用气相色谱/质谱（GC/MS）分析尿类固醇谱已被报道用于诊断新生儿异常类固醇生成，并取得了一些成功。我们试图建立63个日本新生儿尿类固醇的参考值，采用气相色谱/质谱选择离子监测（SIM），每个类固醇使用两个特征质量离子进行最终鉴定。我们研究了36名健康的足月新生儿（1-56日龄）的尿样，以确定参考值（mg/g肌酐、中位数和10-90百分位范围），并探讨白天和夜间肌酐水平的可能差异。我们还研究了23名健康成年女性（20-24岁）的24小时尿液，以比较新生儿的参考值。在不干扰的情况下，测量了每个婴儿的50种代谢物：DHEA、孕烯醇酮、17-羟基孕烯醇酮、雄烯二酮、孕酮、17-羟基孕酮、21-脱氧可的松、皮质酮、18-羟基皮质酮、醛固酮、18-羟基皮质醇、11-脱氧皮质醇、可的松、皮质醇和雌激素，但测量了11-羟基雄烯二酮、孕烯醇酮、11-脱氧皮质酮、皮质酮、11-脱氢皮质酮、21-脱氧皮质醇、由于胎儿皮质类固醇的干扰，每个婴儿的11-脱氧皮质醇和皮质醇无法测量，两个质量离子的峰面积比异常证实了这一点。所有63种代谢物均可在不受干扰的情况下测量。在足月新生儿中，3 -羟基-5-甾体激素的16 -、16 -和15 -羟基代谢物，以及皮质类固醇的6 -、18-羟基和11-氧代谢物的含量明显高于成人。尿类固醇在新生儿中几乎没有昼夜变化，表明斑点尿可以代替24小时尿。
Urinary steroid profile analysis using gas chromatography/mass spectrometry (GC/MS) has been reported for the diagnosis of abnormal steroidogenesis in newborn infants with some success. We tried to establish the reference values of 63 urinary steroids in Japanese newborn infant, using GC/MS in selected ion monitoring (SIM) that utilizes two characteristic mass ions for each steroid for definitive identification. We studied 36 healthy full-term newborn infants (1-56 days of age) on spot urine samples to define the reference values (mg/g creatinine, median and 10-90 percentile range) and to investigate the possible difference between daytime and nighttime levels. We also studied 23 healthy adult females (20-24 years of age) on 24-hour-urine for the comparison of the reference values of newborn infants. Fifty metabolites of DHEA, pregnenolone, 17-hydroxypregnenolone, androstenedione, progesterone, 17-hydroxyprogesterone, 21-deoxycortisone, corticosterone, 18-hydroxycorticosterone, aldosterone, 18-hydroxycortisol, 11-deoxycortisol, cortisone, cortisol, and estrogen in each infant were measurable without interference, but 13 metabolites of 11-hydroxyandrostenedione, pregnenolone, 11-deoxycorticosterone, corticosterone, 11-dehydrocorticosterone, 21-deoxycortisol, 11-deoxycortisol and cortisol were immeasurable in each infant due to the interference of fetal cortex steroids as confirmed by abnormal peak area ratios of two mass ions. All 63 metabolites in each control adult were measurable without interference. 16alpha-, 16beta-, and 15beta-hydroxy metabolites of 3beta-hydroxy-5-en-steroids, and 6beta-, 18-hydroxy and 11-oxo-metabolites of corticosteroids were significantly higher in full-term newborn infants than those in adults as previously reported. Urinary steroids showed little circadian variation in the newborn infants, indicating that spot urine can substitute for 24-hour urine.