Extraction, characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer
Extraction, characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer
复制标题
黄芪多糖的提取、表征及其对胃癌大鼠的免疫调节活性
DOI：
10.1016/j.carbpol.2009.06.005
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发表时间：
2009-11-17
影响因子：
11.2
通讯作者：
Zhang Xue-guang
中科院分区：
文献类型：
--
作者：
Li Rui;Chen Wei-chang;Zhang Xue-guang
利用薄层层析和SephadexG-100柱层析技术，从黄芪多糖提取物中分离得到一个主要的多糖组分--葡萄糖。采用高效液相色谱法和红外光谱法对黄芪多糖进行了定性和定量分析。对HPLC方法的线性、精密度和准确度进行了验证。结果表明，黄芪多糖是一种α-（1 -> 4）-d-葡聚糖，具有α-（1 -> 6）连接的分支，连接在分支点的O-6上。生物活性试验表明，黄芪多糖具有促进脾淋巴细胞增殖的活性。该多糖还具有抗炎活性。这些数据共同表明黄芪多糖具有显著的免疫调节活性，从而支持多糖在胃癌疾病治疗中的广泛使用。(C)2009爱思唯尔有限公司版权所有。
One major polysaccharide fractions, glucose, were isolated from the polysaccharides extract of Astragalus (AP), a valuable traditional Chinese medicine, using thin-layer chromatography (TLC) and Sephadex G-100 chromatography. HPLC and IR methods were used for a qualitative and quantitative determination of from polysaccharides of Astragalus. The HPLC method was validated for linearity, precision and accuracy. The results indicated that polysaccharides of Astragalus is an alpha-(1 -> 4)-d-glucan with alpha-(1 -> 6)-linked branches attached to the O-6 of branch points. Bioactivity tests showed that polysaccharides of Astragolus is active for spleen lymphocytes proliferation. The polysaccharicles also presented anti-inflammatory activities. These data together suggest that polysaccharicles of Astragalus presents significant immune modulating activity, thus supporting the popular use of the polysaccharides in the treatment of gastric cancer diseases. (C) 2009 Elsevier Ltd. All rights reserved.