Phytochemical and biological analysis of Skullcap (Scutellaria lateriflora L.):: A medicinal plant with anxiolytic properties
Phytochemical and biological analysis of Skullcap (Scutellaria lateriflora L.):: A medicinal plant with anxiolytic properties
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DOI：
10.1078/0944-7113-00374
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发表时间：
2003-11-01
期刊：
影响因子：
7.9
通讯作者：
Merali, Z
中科院分区：
文献类型：
--
作者：
Awad, R;Arnason, JT;Merali, Z
研究了黄芩（美国黄芩）的植物化学和生物活性。黄芩传统上用作镇静剂并治疗焦虑等各种神经疾病。进行体内动物行为试验，以测试口服侧叶侧叶草提取物的大鼠的抗焦虑作用。进入“开放场地”中心的人数显着增加；发现了未受保护的低头次数、进入次数以及在高架十字迷宫张开臂上花费的时间长度。使用 HPLC 对 50% EtOH 提取物中的类黄酮、黄芩苷 (40 mg/g) 和 95% EtOH 提取物中的苷元黄芩素 (33 mg/g) 以及 H2O 和 EtOH 提取物中的氨基酸 GABA（类似于 1.6 mg/g）和 H2O 提取物中的谷氨酰胺 (31 mg/g) 进行鉴定和定量。这些化合物可能在抗焦虑活性中发挥作用，因为已知黄芩苷和黄芩素可与 GABA(A) 受体的苯二氮卓位点结合，并且 GABA 是主要的抑制性神经递质。
The phytochemistry and biological activity of Scutellaria lateriflora L. (American skullcap) which has been traditionally used as a sedative and to treat various nervous disorders such as anxiety was studied. In vivo animal behaviour trials were performed to test anxiolytic effects in rats orally administered S. laterifolia extracts. Significant increases in the number of entries into the center of an "open-field arena"; number of unprotected head dips, number of entries and the length of time spent on the open arms of the Elevated Plus-Maze were found. The identification and quantification of the flavonoid, baicalin in a 50% EtOH extract (40 mg/g) and its aglycone baicalein in a 95% EtOH extract (33 mg/g), as well as the amino acids GABA in H2O and EtOH extracts (similar to1.6 mg/g) and glutamine in a H2O extract (31 mg/g), was performed using HPLC. These compounds may play a role in anxiolytic activity since baicalin and baicalein are known to bind to the benzodiazepine site of the GABA(A) receptor and since GABA is the main inhibitory neurotransmitter.