PATCH-CLAMPING OF THE INNER MITOCHONDRIAL-MEMBRANE REVEALS A VOLTAGE-DEPENDENT ION CHANNEL
PATCH-CLAMPING OF THE INNER MITOCHONDRIAL-MEMBRANE REVEALS A VOLTAGE-DEPENDENT ION CHANNEL
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DOI：
10.1038/330498a0
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发表时间：
1987-12-03
期刊：
影响因子：
64.8
通讯作者：
STUHMER, W
中科院分区：
文献类型：
--
作者：
SORGATO, MC;KELLER, BU;STUHMER, W
线粒体的主要功能是为细胞提供三磷酸腺苷（ATP）。 ATP 合成由质子动力 (δp) 驱动，质子动力通过呼吸链的活动在线粒体内膜 (IMM) 上产生并维持1。人们普遍认为，IMM 不太可能包含像质膜中存在的离子通道，因为开放通道的高离子传输特性预计会消散 δp。尽管细胞器的小尺寸阻碍了经典电生理学方法的使用，但最近引入的膜片钳技术允许从非常小的细胞记录电流2，使我们能够检验这一假设。通过膜片钳 IMM，我们发现了一个轻微的阴离子选择性通道，该通道具有电压依赖性，并且在对称 150 mM KCl 存在的情况下具有 107 pS 的平均电导。
The prime function of mitochondria is to provide the cell with adenosine triphosphate (ATP). ATP synthesis is driven by the protonmotive force (δp), which is generated and maintained across the inner mitochondrial membrane (IMM) by the activity of the respiratory chain1. It is widely believed that the IMM is unlikely to contain ion channels like those present in the plasma membrane, because the high rates of ion transport characteristic of open channels would be expected to dissipate the δp. Although the small size of the organelle has prevented the use of classical electrophysiological methods, the recent introduction of the patch-clamp technique, which allows currents to be recorded from very small cells2, has enabled us to test this hypothesis. By patch-clamping the IMM, we have identified a slightly anion-selective channel, which is voltage-dependent and has a mean conductance of 107 pS in the presence of symmetrical 150 mM KCl.