Immunohistochemical distribution of alpha-neo-endorphin/dynorphin neuronal systems in rat brain: evidence for colocalization.
Immunohistochemical distribution of alpha-neo-endorphin/dynorphin neuronal systems in rat brain: evidence for colocalization.
复制标题
大鼠脑中α-新内啡肽/强啡肽神经元系统的免疫组织化学分布：共定位的证据。
DOI：
10.1073/pnas.79.9.3062
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发表时间：
1982
影响因子：
11.1
通讯作者：
Barchas,JD
中科院分区：
文献类型：
--
作者：
Weber,E;Roth,KA;Barchas,JD
采用冷冻切片双抗体免疫荧光法观察大鼠脑内α -新内啡肽和强啡肽的免疫反应。使用的抗体对各自的抗原具有特异性。在相邻的连续切片中，α -新内啡肽和肌啡肽抗血清产生的神经元免疫染色模式是完全不可能重叠的。未发现单独存在α -新内啡肽或肌啡肽免疫反应的区域。以下脑区含有α -新内啡肽/啡肽免疫反应纤维和终末：前脑正中束、内囊、黑质、下丘脑、伏隔核、海马和延髓。在大脑皮层和纹状体中可见少量纤维。在许多区域，这种神经纤维系统似乎与先前描述的含有[Met]-/[Leu]脑啡肽免疫反应物质的神经系统重叠。在秋水仙碱处理过的动物的大脑中，在下丘脑的视上核、视交叉后核、室旁核和大细胞副核中可见大量的α -新内啡肽/啡肽免疫反应神经元胞体。由此可见，α -新内啡肽样免疫反应和强啡样免疫反应是同一神经系统的一部分。
alpha-Neo-endorphin and dynorphin immunoreactivities in rat brain were visualized by double antibody immunofluorescence of frozen sections. Antibodies were used that were specific for their respective antigens. The pattern of neuronal immunostaining produced by alpha-neo-endorphin and dynorphin antisera in adjacent serial sections was completely superimposible. No areas were found in which alpha-neo-endorphin or dynorphin immunoreactivities existed alone. The following brain regions contained alpha-neo-endorphin/dynorphin-immunoreactive fibers and terminals: the median forebrain bundle, the internal capsule, the substantia nigra, the hypothalamus, the nucleus accumbens, the hippocampus, and the medulla oblongata. A few fibers were seen in the cerebral cortex and in the corpus striatum. In many regions, this neuronal fiber system seems to overlap the neuronal system previously described to contain [Met]-/[Leu]enkephalin-immunoreactive material. In brains from colchicine-treated animals, numerous alpha-neo-endorphin/dynorphin-immunoreactive neuronal cell bodies were seen in the supraoptic, retrochiasmatic supraoptic, paraventricular, and magnocellular accessory nuclei of the hypothalamus. It is concluded that alpha-neo-endorphin-like and dynorphin-like immunoreactivities are part of the same neuronal system.