Solid-state NMR analysis of the orientation and dynamics of epigallocatechin gallate, a green tea polyphenol, incorporated into lipid bilayers
Solid-state NMR analysis of the orientation and dynamics of epigallocatechin gallate, a green tea polyphenol, incorporated into lipid bilayers
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DOI：
10.1002/mrc.2157
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发表时间：
2008-02-01
影响因子：
2
通讯作者：
Nakayama, Tsutomu
中科院分区：
文献类型：
--
作者：
Kajiya, Katsuko;Kumazawa, Shigenori;Nakayama, Tsutomu
儿茶素是绿茶中主要的多酚类化合物，已鉴定的四种主要化合物是表儿茶素(EC)、表儿茶素(EGC)、表儿茶素没食子酸酯(CG)和表没食子儿茶素没食子酸酯(EGCG)。茶儿茶素倾向于在外部附着在其靶标上，如病毒膜、细胞膜或低密度脂蛋白的表面。为了进一步了解这些化合物在细胞中的分子流动性，我们利用固体核磁共振技术研究了茶儿茶素与脂膜的相互作用。我们以前的工作表明，EGCG分子以独特的方向被掺入到脂质双层中。然而，EGCG在脂质双层中的详细结构、取向和动力学还没有得到很好的描述。在这里，我们用固体核磁共振波谱研究了EGCG在多层囊泡(MLV)和双分子囊泡中的取向和动力学。版权所有(C)2007 John Wiley&Sons，Ltd.
Catechins are the principle polyphenolic compounds in green tea; the four major compounds identified are epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg). Tea catechins tend to attach externally to their targets, such as viral envelopes, cell membranes, or the surface of low-density lipoproteins. In order to further our understanding of the molecular mobility of these compounds in cells, we examined the interaction of tea catechins with lipid membranes using solid-state NMR techniques. Our previous work indicated that the EGCg molecule is incorporated into lipid bilayers in a unique orientation. However, the detailed configuration, orientation, and dynamics of EGCg in lipid bilayers have not been well-characterized. Here, we investigated the orientation and dynamics of EGCg incorporated into multi-lamellar vesicles (MLVs) and bicelles using solid-state NMR spectroscopy. Copyright (c) 2007 John Wiley & Sons, Ltd.