Effect of leukemia inhibitory factor in experimental cisplatin neuropathy in mice
Effect of leukemia inhibitory factor in experimental cisplatin neuropathy in mice
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DOI：
10.1016/j.cyto.2004.09.006
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发表时间：
2005-01-07
期刊：
影响因子：
3.8
通讯作者：
Taspinar, M
中科院分区：
文献类型：
--
作者：
Öztürk, G;Erdogan, E;Taspinar, M
在这项研究中，白血病抑制因子（LIF）对顺铂（CDDP）诱导的神经病变的影响进行了评估。用CDDP（2 mg/kg，腹膜内，每周两次，共九次）处理小鼠。在最后一周，还向一些小鼠皮下注射LIF，2 μ g/kg。每隔一天注射一次，总共注射四次。通过甩尾潜伏期和感觉神经传导速度（NCV）的变化来评估神经病变的发展。在给药期结束时，对背根神经节（DRG）进行显微镜检查。将部分DRG植入细胞外基质中，用培养基覆盖并孵育3天。在孵育期间和孵育结束时，定量从DRG的细胞迁移和轴突生长。LIF可逆转CDDP引起的甩尾潜伏期延长（p < 0.05），并可改善CDDP引起的NCV降低。CDDP使神经元的核和体细胞体积减小，而LIF使后者恢复到对照值（p
In this study, the effect of leukemia inhibitory factor (LIF) on cisplatin (CDDP)-induced neuropathy was evaluated. Mice were treated with CDDP, 2 mg/kg i.p. twice a week nine times. During the last week some of the mice were also injected with LIF, 2 mug/kg s.c. every other day for a total of four injections. Development of neuropathy was evaluated with changes in tail flick latency and sensory nerve conduction velocity (NCV). At the end of the treatment period dorsal root ganglia (DRG) were microscopically examined. Some of the DRGs were explanted into extracellular matrix, covered with culture medium and incubated for 3 days. During and at the end of the incubation, cellular migration and axonal outgrowth from the DRGs were quantified. LIF proved effective in reversing the increase in tail flick latency (p < 0.05) and improving the reduction in NCV induced by CDDP. CDDP led to smaller nuclear and somatic size in neurons, while with LIF, the latter was restored to control values (p