Transforming growth factor-β inhibits nephronectin-induced osteoblast differentiation
Transforming growth factor-β inhibits nephronectin-induced osteoblast differentiation
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DOI：
10.1016/j.febslet.2010.04.074
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发表时间：
2010-07-02
期刊：
影响因子：
3.5
通讯作者：
Yang, Burton B.
中科院分区：
文献类型：
--
作者：
Fang, Ling;Kahai, Shireen;Yang, Burton B.
我们使用cDNA微阵列来鉴定成骨细胞发育过程中转化生长因子β（TGF-β）反应性靶基因，发现肾连蛋白（Npnt）是一个显著下调的基因。在这里，我们报告的作用，TGF-β调节Npnt介导的成骨细胞分化。我们发现TGF-β对Npnt表达的影响与细胞形态的变化呈剂量依赖性相关。Npnt诱导的成骨细胞分化也受到TGF-β的抑制，TGF-β使细胞形态从立方变为成纤维细胞，这表明成骨细胞分化被破坏。此外，TGF-β抑制用各种截短的Npnt构建体转染的成骨细胞的分化，表明TGF-β可以对Npnt功能发挥下游作用。我们的研究结果表明，TGF-β可以通过多种机制抑制成骨细胞分化。(C)2010年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
We used cDNA microarray to identify transforming growth factor beta (TGF-beta) responsive target genes during osteoblast development and found that nephronectin (Npnt) is one such gene that is significantly down-regulated. Here we report the role of TGF-beta in regulating Npnt-mediated osteoblast differentiation. We found that the effect of TGF-beta on Npnt expression is associated with a change in cell morphology in a dose-dependent manner. Npnt-induced osteoblast differentiation was also inhibited by TGF-beta, which changed cell morphology from cuboidal to fibroblastic, an indication that osteoblast differentiation was disrupted. Furthermore, TGF-beta inhibited differentiation of osteoblasts transfected with various truncated Npnt constructs, suggesting that TGF-beta can exert a down-stream effect on Npnt function. Our results suggest that TGF-beta can inhibit osteoblast differentiation through various mechanisms. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.