Stepwise selection of natural variations at CTB2 and CTB4a improves cold adaptation during domestication of japonica rice
Stepwise selection of natural variations at CTB2 and CTB4a improves cold adaptation during domestication of japonica rice
复制标题
CTB2和CTB4a自然变异的逐步选择提高了粳稻驯化过程中的冷适应能力
DOI：
10.1111/nph.17407
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发表时间：
2021-06-10
期刊：
影响因子：
9.4
通讯作者：
Li, Zichao
中科院分区：
文献类型：
--
作者：
Li, Jilong;Zeng, Yawen;Li, Zichao
水稻冷适应性的提高有助于水稻种植面积的增加。在植物适应过程中，持续变异和从头突变是有益等位基因的两种不同的自然来源。然而，在作物驯化过程中，这些来源在一个单一的人口背后的遗传机制和进化模式仍然habitive. There我们克隆CTB 2基因，编码UDP-葡萄糖甾醇葡萄糖基转移酶，在水稻孕穗期耐冷。CTB 2保守的UDPGT结构域中存在一个单一的变异（I408 V），该变异起源于中国普通野生稻（Oryza rufipogon），与水稻（Oryza sativa ssp.）CTB 2位于56.8kb的区域内，包括先前报道的基因CTB 4a，其中在c.中国云南省距今3200年左右，具有耐寒性。CTB 2的长期变异和CTB 4a的新突变通过逐步选择来促进冷适应，从而使水稻从高海拔地区向高纬度地区推广种植，这些结果为两种变异的逐步选择提供了一个范例，并描述了一种新的粳稻冷适应分子机制。
The improvement of cold adaptation has contributed to the increased growing area of rice. Standing variation and de novo mutation are distinct natural sources of beneficial alleles in plant adaptation. However, the genetic mechanisms and evolutionary patterns underlying these sources in a single population during crop domestication remain elusive.Here we cloned the CTB2 gene, encoding a UDP-glucose sterol glucosyltransferase, for cold tolerance in rice at the booting stage. A single standing variation (I408V) in the conserved UDPGT domain of CTB2 originated from Chinese Oryza rufipogon and contributed to the cold adaptation of Oryza sativa ssp. japonica.CTB2 is located in a 56.8 kb region, including the previously reported gene CTB4a in which de novo mutation arose c. 3200 yr BP in Yunnan province, China, conferring cold tolerance. Standing variation of CTB2 and de novo mutation of CTB4a underwent stepwise selection to facilitate cold adaptation to expand rice cultivation from high-altitude to high-latitude regions.These results provide an example of stepwise selection on two kinds of variation and describe a new molecular mechanism of cold adaptation in japonica rice.