Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells
Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells
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DOI：
10.1016/j.canlet.2011.04.009
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发表时间：
2011-11-01
期刊：
影响因子：
9.7
通讯作者：
Maffia, Michele
中科院分区：
文献类型：
--
作者：
Vergara, Daniele;Valente, Concetta Maria;Maffia, Michele
癌进展与上皮特征的丧失以及肿瘤细胞获得间充质表型相关。在本文中，我们表明MCF-7细胞暴露于表皮生长因子（EGF）会导致上皮向间充质转化（EMT）特征的形态学改变。EGF处理后，MCF-7细胞的运动性沿着增加，同时转录因子Slug、Zeb 1、Zeb 2和间充质标志物Vimentin和N-cadherin.Treatment与EGF和白藜芦醇（一种天然存在的具有抗肿瘤特性的二苯乙烯）的联合刺激未能改变细胞形态、运动性和EGF诱导的EMT标志物的过表达。本研究通过使用特异性化学抑制剂，证实EGF诱导的EMT是通过细胞外信号调节激酶1/2（ERK 1/2）信号通路介导的，白藜芦醇能够抑制EGF诱导的ERK活化，为白藜芦醇抑制EGF诱导的EMT细胞转化提供了新的证据。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Carcinoma progression is associated with the loss of epithelial features, and the acquisition of a mesenchymal phenotype by tumour cells. Herein we show that exposure of MCF-7 cells to epidermal growth factor (EGF) resulted in morphological alterations characteristic of epithelial-to-mesenchymal transition (EMT). EGF treatment resulted in increased motility along with an up-regulation of transcription factors Slug, Zeb1, Zeb2, and mesenchymal markers Vimentin and N-cadherin.Treatment of MCF-7 cells with a combined stimulation of EGF and resveratrol, a naturally occurring stilbene with antitumor properties, failed to alter cell morphology, motility and overexpression of EMT markers induced by EGF. Using specific chemical inhibitors, we demonstrated that EGF-induced EMT is mediated by extracellular signal-regulated kinase 1/2 (ERK 1/2) signalling pathway and that resveratrol is able to repress EGF-induced ERK activation.In summary, these data provide new evidence of the inhibitory effect of resveratrol on EGF-induced EMT cell transformation. (C) 2011 Elsevier Ireland Ltd. All rights reserved.