Hepatic and fecal metabolomic analysis of the effects of Lactobacillus rhamnosus GG on alcoholic fatty liver disease in mice.
Hepatic and fecal metabolomic analysis of the effects of Lactobacillus rhamnosus GG on alcoholic fatty liver disease in mice.
复制标题
乳糖乳杆菌GG对小鼠酒精脂肪肝病的影响的肝和粪便代谢组学分析。
DOI：
10.1021/pr501121c
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发表时间：
2015-02-06
影响因子：
4.4
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Shi X;Wei X;Yin X;Wang Y;Zhang M;Zhao C;Zhao H;McClain CJ;Feng W;Zhang X
肠道、肝脏和免疫系统之间的相互作用在肝脏疾病中起着重要作用。益生菌已被用于治疗和预防许多病理条件，包括肝脏疾病。本文使用全面的二维气相色谱飞行时间质谱（GC× GC-TOF 0020 MS）结合化学计量学数据分析来鉴定喂食或不喂食酒精的小鼠中受益生菌显著影响的代谢物。代谢组学分析表明，当小鼠长期暴露于酒精时，小鼠肝脏中的脂肪酸水平增加，而小鼠粪便中的脂肪酸水平降低。用来自鼠李糖乳杆菌GG（Lactobacillus rhamnosus GG，LGGs）的培养上清液补充酒精喂养的小鼠使这些酒精诱导的异常正常化，并预防酒精性肝病（ALD）。这些结果与以前的研究一致。除了饮食来源的长链脂肪酸（LCFA）外，LGG还可以积极地改变肠道细菌种群，刺激LCFA合成，这已被证明可以增强肠道屏障功能，减少内毒素血症，并预防ALD。我们还发现，几种氨基酸，包括L-异亮氨酸，支链氨基酸，在暴露于酒精的动物的肝脏和粪便样本中下调，这些氨基酸的水平被LGG校正。这些结果表明，LGGs通过增加肠脂肪酸和减少肝脂肪酸以及增加氨基酸浓度来减轻酒精诱导的脂肪肝。
The interactions among the gut, liver, and immune system play an important role in liver disease. Probiotics have been used for the treatment and prevention of many pathological conditions, including liver diseases. Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOF0020MS) was used herein, in conjunction with chemometric data analysis, to identify metabolites significantly affected by probiotics in mice fed with or without alcohol. The metabolomics analysis indicates that the levels of fatty acids increased in mouse liver and decreased in mouse feces when mice were chronically exposed to alcohol. Supplementing the alcohol-fed mice with culture supernatant from Lactobacillus rhamnosus GG (LGGs) normalized these alcohol-induced abnormalities and prevented alcoholic liver disease (ALD). These results agree well with previous studies. In addition to diet-derived long chain fatty acids (LCFAs), LGGs may positively modify the gut’s bacterial population to stimulate LCFA synthesis, which has been shown to enhance intestinal barrier function, reduce endotoxemia, and prevent ALD. We also found that several amino acids, including L-isoleucine, a branched chain amino acid, were downregulated in the liver and fecal samples from animals exposed to alcohol and that the levels of these amino acids were corrected by LGGs. These results demonstrate that LGGs alleviates alcohol-induced fatty liver by mechanisms involving increasing intestinal and decreasing hepatic fatty acids and increasing amino acid concentration.
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影响因子：
7.4
作者：
Koo, Imhoi;Zhang, Xiang;Kim, Seongho
通讯作者：
Kim, Seongho
影响因子：
29.4
作者：
Chen P;Torralba M;Tan J;Embree M;Zengler K;Stärkel P;van Pijkeren JP;DePew J;Loomba R;Ho SB;Bajaj JS;Mutlu EA;Keshavarzian A;Tsukamoto H;Nelson KE;Fouts DE;Schnabl B
通讯作者：
Schnabl B
DOI：
10.1016/j.alcohol.2008.12.009
发表时间：
2009-03
期刊：
Alcohol (Fayetteville, N.Y.)
影响因子：
--
作者：
Forsyth CB;Farhadi A;Jakate SM;Tang Y;Shaikh M;Keshavarzian A
通讯作者：
Keshavarzian A
DOI：
10.1016/j.jnutbio.2013.02.001
发表时间：
2013-09
期刊：
The Journal of nutritional biochemistry
影响因子：
--
作者：
Wang Y;Liu Y;Kirpich I;Ma Z;Wang C;Zhang M;Suttles J;McClain C;Feng W
通讯作者：
Feng W
影响因子：
4.2
作者：
Charlton, M
通讯作者：
Charlton, M