Cytoskeletal control of myogenesis: a desmin null mutation blocks the myogenic pathway during embryonic stem cell differentiation.
Cytoskeletal control of myogenesis: a desmin null mutation blocks the myogenic pathway during embryonic stem cell differentiation.
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肌生成的细胞骨架控制：结蛋白无效突变阻断胚胎干细胞分化过程中的肌生成途径。
DOI：
10.1006/dbio.1995.8070
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Capetanaki,Y
中科院分区：
文献类型：
--
作者：
Weitzer,G;Milner,DJ;Kim,JU;Bradley,A;Capetanaki,Y
建立了一个基于胚胎干细胞衍生的胚状体（EBs）的分化系统，用于研究结蛋白基因的破坏对肌细胞分化的影响。评估了具有突变的结蛋白等位基因的野生型、杂合和纯合细胞系。结蛋白无效突变EBs的骨骼肌发生被完全抑制，表现为肌管形成，收缩性和myoD，肌细胞生成素，myf5和肌球蛋白重链表达的情况下。在结蛋白不存在的情况下，平滑肌形成也被完全阻断。另一方面，这些结蛋白无效突变EBs对心肌细胞分化没有明显影响。然而，减少结蛋白表达的EB杂合子的结蛋白突变导致心肌形成的部分抑制。这些数据表明，在早期心肌细胞分化相反，结蛋白是必不可少的骨骼肌和平滑肌的形成。
A differentiating system based on embryonic stem (ES) cell-derived embryoid bodies (EBs) which recapitulates thein vivocardiac, skeletal, and smooth muscle myogenesis of mouse embryos was developed and used to investigate the effects of the disruption of the desmin gene on muscle cell differentiation. Wild-type, heterozygous, and homozygous cell lines with the mutated desmin allele were evaluated. Skeletal myogenesis was totally inhibited in desmin null mutant EBs, as manifested by the absence of myotube formation, contractility, and myoD, myogenin, myf5, and myosin heavy chain expression. Smooth muscle formation was also completely blocked in the absence of desmin. On the other hand, there were no obvious effects on cardiomyocyte differentiation in these desmin null mutant EBs. However, reduced desmin expression in EBs heterozygous for the desmin mutation leads to partial inhibition of cardiac muscle formation. These data suggest that in contrast to early cardiocyte differentiation, desmin is indispensable for skeletal and smooth muscle formation.