103Ruthenium red, a reagent for detecting glycosaminoglycans at the nanogram level.
103Ruthenium red, a reagent for detecting glycosaminoglycans at the nanogram level.
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103钌红，一种用于检测纳克级糖胺聚糖的试剂。
DOI：
10.1016/0003-2697(82)90296-2
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发表时间：
1982
影响因子：
2.9
通讯作者：
Carlson,SS
中科院分区：
文献类型：
--
作者：
Carlson,SS
使用一种新的试剂103钌红，可以在醋酸纤维素电泳图上检测糖胺聚糖，其灵敏度比目前可用的其他方法高得多。非放射性的钌红在过去被广泛用作多阴离子的电子显微镜染色剂。商业上可用的103rucl3可以很容易地转化为103钌红，这种β粒子发射同位素可以用放射自显影术检测到。肝素、硫酸肝素或硫酸软骨素的检出限为2-4 ng。
Using a new reagent,103ruthenium red, glycosaminoglycans can be detected on cellulose acetate electrophoretograms at much greater sensitivity than with other methods currently available. Nonradioactive ruthenium red has been used extensively in the past as an electron microscopic stain for polyanions. Commercially available103RuCl3can be easily converted to103ruthenium red and this beta-particle-emitting isotope can be detected with autoradiography. The limit of detection for heparin, heparan sulfate, or chondroitin sulfate is 2–4 ng.