Multi-omics analysis reveals the mechanism of torularhodin accumulation in the mutant Rhodosporidium toruloides A1-15 under nitrogen-limited conditions.
Multi-omics analysis reveals the mechanism of torularhodin accumulation in the mutant Rhodosporidium toruloides A1-15 under nitrogen-limited conditions.
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DOI：
10.1039/d3fo01097j
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发表时间：
2023-06
期刊：
影响因子：
6.1
通讯作者：
Mengyang Liu;Haitao Wang;Xu Yan;Sufang Zhang;Chaofan Ji;Yingxi Chen;B. Zhu;Xinping Lin
中科院分区：
文献类型：
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作者：
Mengyang Liu;Haitao Wang;Xu Yan;Sufang Zhang;Chaofan Ji;Yingxi Chen;B. Zhu;Xinping Lin
类胡萝卜素产生菌红冬孢酵母（Rhodosporidium toruloides）NP 11及其突变株R.在恒化器氮限制培养条件下，对圆红酵母A1 - 15进行了研究。采用多组学分析方法（代谢组学、脂质组学和转录组学）研究了NP11和A1 - 15之间红曲霉素积累的不同机制。结果表明，在氮素限制条件下，A1 - 15的类胡萝卜素合成途径比NP11显著增强，这是由于红曲霉红色素的显著增加。在氮限制条件下，与NP 11相比，A1 - 15中存在更高水平的β-氧化，这为类胡萝卜素的合成提供了足够的前体。此外，ROS胁迫促进了铁离子的胞内转运，促进了CRTI和CRTY基因的表达，降低了旁路途径中FNTB1和FNTB2的转录水平，这些因素可能是A1 - 15高产量圆棒紫红素的调控因素。本研究为圆叶红景天素的选择性生产提供了新的思路。
A carotenoid production strain Rhodosporidium toruloides NP11 and its mutant strain R. toruloides A1-15 were studied under chemostat nitrogen-limited cultivation. Multi-omics analysis (metabolomics, lipidomics and transcriptomics) was used to investigate the different mechanisms of torularhodin accumulation between NP11 and A1-15. The results showed that the carotenoid synthesis pathway was significantly enhanced in A1-15 compared to NP11 under nitrogen limitation, due to the significant increase of torularhodin. Under nitrogen-limited conditions, higher levels of β-oxidation were present in A1-15 compared to those in NP11, which provided sufficient precursors for carotenoid synthesis. In addition, ROS stress accelerated the intracellular transport of iron ions, promoted the expression of CRTI and CRTY genes, and reduced the transcript levels of FNTB1 and FNTB2 in the bypass pathway, and these factors may be responsible for the regulation of high torularhodin production in A1-15. This study provided insights into the selective production of torularhodin.