Dorsal fin development in flounder, Paralichthys olivaceus: Bud formation and its cellular origin
Dorsal fin development in flounder, Paralichthys olivaceus: Bud formation and its cellular origin
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牙鲆的背鳍发育：芽的形成及其细胞起源
DOI：
10.1016/j.gep.2017.04.003
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发表时间：
2017-11-01
影响因子：
1.2
通讯作者：
Bao, Baolong
中科院分区：
文献类型：
--
作者：
Chen, Jie;Liu, Xiaoyu;Bao, Baolong
中鳍的发育在硬骨鱼类中没有得到广泛的研究，尽管在其他鱼类中，有人提出它涉及与成对附肢相同的遗传程序。成虫的中鳍是由幼虫的芽发育而来的，因此研究鳍芽的形成及其细胞起源对于了解其成熟机制至关重要。在褐牙鲆中，骨骼发生是由前向后的，这为研究背鳍芽的形成提供了良好的机会。在孵化后第3天，在假定背鳍的基底层首次观察到顶端外胚层脊。孵化后6天，随着芽的长出，顶端外胚层褶开始形成。芽继续生长，在孵化后9天的幼虫突破背鳍褶皱。在孵化后13天，芽生长超过鳍褶的边缘，并形成四个未来的射线。细胞类型的分子标记显示，背鳍芽中存在神经嵴细胞、成骨细胞和核细胞。Hoxd10基因在仔鱼背鳍芽中的表达最早，其次为Hoxd9、Hoxd11和Hoxd12。这表明Hoxd 10可能是芽形成位点的候选分子标记。一些关键的分子标记，如FGF8，Wnt7，和Shh的配对附属器的发展，在顶端外胚层嵴，后来顶端外胚层褶皱。Hh通路抑制剂可抑制背鳍芽的形成，进一步表明中鳍可能利用了共同的基本分子机制。(C)2017 Elsevier B.V.版权所有。
The development of the median fin has not been investigated extensively in teleosts, although in other fishes it has been proposed that it involves the same genetic programs operating in the paired appendages. Adult median fins develop from the larval bud; therefore an investigation of fin bud formation and its cellular origin is essential to understanding the maturation mechanisms. In Paralichthys olivaceus, skeletogenesis proceeds from an anterior to posterior direction providing a good opportunity to study the formation of dorsal fin bud. An apical ectodermal ridge appeared at the basal stratum of the presumptive dorsal fin was first observed at 3 days post hatching. Then the apical ectodermal fold formed as the bud outgrew in 6 days post-hatch larvae. The bud continued to grow, breaking through the dorsal fin fold in 9 days post-hatch larvae. At 13 days post-hatch, the bud grew beyond the edge of the fin fold and formed into the four future rays. Molecular markers of cell type showed the existence of neural crest cells, scleroblasts and sclerotomes in the dorsal fin bud. The earliest gene expression in the dorsal fin bud was Hoxd10 at 3 days post-hatch larvae, then Hoxd9, Hoxdll and Hoxd12. This indicates Hoxd10 might be a candidate molecular marker of the bud formation site. Some key molecular markers for paired appendage development, such as FGF8, Wnt7, and Shh were expressed at the apical ectodermal ridge and later the apical ectodermal fold. Moreover, the form of the dorsal fin bud could be inhibited by Hh pathway inhibitor, further indicating that common basic molecular mechanisms might be utilized by median fins. (C) 2017 Elsevier B.V. All rights reserved.