Effects of various spacers between biotin and the phospholipid headgroup on immobilization and sedimentation of biotinylated phospholipid-containing liposomes facilitated by avidin-biotin interactions.
Effects of various spacers between biotin and the phospholipid headgroup on immobilization and sedimentation of biotinylated phospholipid-containing liposomes facilitated by avidin-biotin interactions.
复制标题
生物素和磷脂头基之间的各种间隔物对亲和素-生物素相互作用促进的生物素化含磷脂脂质体的固定和沉淀的影响。
DOI：
10.1093/jb/mvx016
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发表时间：
2017
影响因子：
2.7
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Sakamoto;Y.;Kikuchi;K.;Umeda;K. & Nakanishi;H.
脂质体（脂质囊泡）的固定和沉淀用于脂质体-蛋白质结合测定，由抗生物素蛋白/链霉抗生物素蛋白/NeutrAvidin 和生物素化的含磷脂的脂质体促进。在这里，我们研究了生物素和磷脂头基之间的三种间隔基[六碳 (X)、聚乙二醇 (PEG) 180（分子量 180）和 PEG2000（分子量 2,000）] 对小单层脂质体/囊泡 (SUV) 的固定和沉淀的影响。 PEG180 和 PEG2000 显示出比 X 更有效地将生物素化 SUV 固定在 NeutrAvidin 包被的板上，但 X 和 PEG180 在添加 NeutrAvidin 后显示出比 PEG2000 更有效的生物素化 SUV 沉降。因此，最合适的垫片在固定和沉淀之间有所不同。生物素化 SUV 的间隔基必须根据所检查的特定脂质体-蛋白质结合测定来选择。
Immobilization and sedimentation of liposomes (lipid vesicles) are used in liposome-protein binding assays, facilitated by avidin/streptavidin/NeutrAvidin and biotinylated phospholipid-containing liposomes. Here, we examined the effects of three spacers [six-carbon (X), polyethylene glycol (PEG) 180 (molecular weight 180) and PEG2000 (molecular weight 2,000)] between biotin and the phospholipid headgroup on the immobilization and sedimentation of small unilamellar liposomes/vesicles (SUVs). PEG180 and PEG2000 showed more efficient immobilization of biotinylated SUVs on NeutrAvidin-coated plates than X, but X and PEG180 showed more efficient sedimentation of biotinylated SUVs upon NeutrAvidin addition than PEG2000. Thus, the most appropriate spacers differed between immobilization and sedimentation. A spacer for biotinylated SUVs must be selected according to the particular liposome-protein binding assays examined.