EXPRESSION OF INTERMEDIATE FILAMENT PROTEINS IN NORMAL AND DISEASED THYROID-GLANDS
EXPRESSION OF INTERMEDIATE FILAMENT PROTEINS IN NORMAL AND DISEASED THYROID-GLANDS
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DOI：
10.1136/jcp.40.2.136
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发表时间：
1987-02-01
影响因子：
3.4
通讯作者：
MASON, DY
中科院分区：
文献类型：
--
作者：
BULEY, ID;GATTER, KC;MASON, DY
用一组针对中间丝蛋白的单克隆抗体对来自正常和病理性甲状腺的总共67个样品进行染色（作为福尔马林固定的石蜡切片）。这项研究证实了以前的报道，即细胞角蛋白和波形蛋白在原发性甲状腺癌中共表达，但在正常甲状腺和一系列良性疾病中也检测到共表达，包括滤泡性腺瘤、桥本甲状腺炎和弥漫性增生（甲状腺毒症）。前角蛋白表达被发现（使用抗体识别高分子量的细胞角蛋白）主要在鳞状变化的领域，独立的基础甲状腺病理。因此，这项研究并不支持之前的发现，即前角蛋白表达提供了区分滤泡型乳头状癌与预后较差的滤泡癌的可靠方法，或者它有助于区分良性和恶性乳头状病变。没有证据表明结蛋白或神经丝蛋白的表达，特别是，神经丝蛋白不能检测到任何髓样癌的情况下研究。
A total of 67 samples from normal and patholoical thyroid glands were stained (as formalin fixed paraffin sections) with a panel of monoclonal antibodies directed against intermediate filament proteins. The study confirmed previous reports of cytokeratin and vimentin coexpression in primarily thyroid carcinomas, but coexpression was also detected in normal thyroid and in a range of benign conditions including follicular adenomas, Hashimoto''s thyroiditis, and diffuse hyperplasia (thyrotoxicosis). Prekeratin expression was found (using antibodies recognising higher molecular weight cytokeratins) predominantly in areas of squamous change, independent of the underlying thyroid pathology. This study does not therefore support previous findings that prekeratin expression provides a reliable means of distinguishing follicular pattern papillary carcinoma from follicular carcinoma with its poorer prognosis or that it helps distinguish benign from malignant papilary lesions. No evidence of desmin or neurofilament expression was seen, and in particular, neurofilaments could not be detected in any of the cases of medullary carcinoma studied.