Discrimination among multiple AATAAA sequences correlates with interspecies conservation of select 3' untranslated nucleotides.
Discrimination among multiple AATAAA sequences correlates with interspecies conservation of select 3' untranslated nucleotides.
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多个 AATAAA 序列之间的区分与所选 3 非翻译核苷酸的种间保守性相关。
DOI：
10.1093/nar/14.11.4499
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发表时间：
1986
影响因子：
14.9
通讯作者：
Gudas,LJ
中科院分区：
文献类型：
--
作者：
Myers,JC;Brinker,JM;Kefalides,NA;Rosenbloom,J;Wang,SY;Gudas,LJ
测定了6.5 kb人原胶原α1(IV) mRNA 1.3 kb 3‘未翻译区对应的DNA序列，并与从3’ cDNA和基因组克隆中获得的小鼠序列进行了比较(Oberbaumer et al., 1985, Eur。[j] .生物化学杂志。147:217)。虽然在人类rna和小鼠rna中分别发现了4个AAUAAA六核苷酸，但Northern blot杂交显示，与使用αl(I)、α2(I)、αl（III）和α2(V)前胶原探针时所看到的模式相反，大多数3'序列几乎被完全利用。此外，在主要αl(IV) mrna中，与包含其他三个共享AAUAAA识别信号的核苷酸相比，聚A tall 1上的90个核苷酸5′具有更大程度的种间同源性。对这一高度保守区域的进一步研究显示，在许多RNA聚合酶II转录基因的3‘非编码区发现了两个“一致序列”（Mattaj and Zeller, 1983, Embo J. 2:1883），出乎意料的是，与其他前胶原mrna的聚a附着信号的核苷酸5’有一些相似性。
The DNA sequence corresponding to the 1.3 kb 3' untranslated region of the 6.5 kb human procoilagen α1(IV) mRNA was determined and compared with the mouse sequence obtained from 3' cDNA and genomic clones overlapping the reported 5' half (Oberbaumer et al., 1985, Eur. J. Biochem. 147:217). Although four AAUAAA hexanucleotides are found In the human and seven 1n the mouse RNAs, Northern blot hybridization showed almost exclusive utilization of the most 3' sequence, in contrast to the pattern seen when using αl(I), α2(I), αl(III) and α2(V) procollagen probes. Moreover, the ninety nucleotides 5' to the poly A tall 1n the major αl(IV) mRNAs exhibit a much greater degree of interspecies homology than those encompassing the other three shared AAUAAA recognition signals. Further examination of this highly conserved area revealed the presence of two “consensus sequences” found 1n the 3' noncoding region of a number of RNA polymerase II transcribed genes (Mattaj and Zeller, 1983, Embo J. 2:1883) and, unexpectedly, some similarity with the nucleotides 5' to the poly A attachment signals 1n other procollagen mRNAs.