Charge state coalescence during electrospray ionization improves peptide identification by tandem mass spectrometry.
Charge state coalescence during electrospray ionization improves peptide identification by tandem mass spectrometry.
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DOI：
10.1007/s13361-012-0404-0
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发表时间：
2012-08
影响因子：
3.2
通讯作者：
A Komives E
中科院分区：
文献类型：
--
作者：
Meyer JG;A Komives E
我们报告增压试剂二甲基亚砜（DMSO）和间硝基苯甲醇（m-NBA）应用于非靶向肽鉴定的效果，特别强调非胰蛋白酶肽。从用胰蛋白酶、弹性蛋白酶或胃蛋白酶消化的五种标准蛋白质的混合物产生的肽用纳流液相色谱法分离，所述纳流液相色谱法使用用5% DMSO或0. 1% m-NBA修饰的移动的相。通过在线电喷雾电离洗脱肽，并使用数据依赖性MS/MS通过CID和ETD两者进行测序。用DMSO共溶剂观察到肽鉴定的统计学显著改善。为了理解这一观察结果，我们评估了增压试剂对色谱分离和电喷雾质量的影响。鉴别的增加不是由于增压，其对于0.1% m-NBA共溶剂更大，而对于5.0% DMSO共溶剂未观察到。使用DMSO改性的移动的相的MS/MS效率的提高似乎是由于电荷状态聚结。
We report the effects of supercharging reagents dimethyl sulphoxide (DMSO) and m-nitrobenzyl alcohol (m-NBA) applied to untargeted peptide identification, with special emphasis on nontryptic peptides. Peptides generated from a mixture of five standard proteins digested with trypsin, elastase, or pepsin were separated with nanoflow liquid chromatography using mobile phases modified with either 5 % DMSO or 0.1 %m-NBA. Eluting peptides were ionized by online electrospray and sequenced by both CID and ETD using data-dependent MS/MS. Statistically significant improvements in peptide identifications were observed with DMSO co-solvent. In order to understand this observation, we assessed the effects of supercharging reagents on the chromatographic separation and the electrospray quality. The increase in identifications was not due to supercharging, which was greater for the 0.1 %m-NBA co-solvent and not observed for the 5.0 % DMSO co-solvent. The improved MS/MS efficiency using the DMSO modified mobile phase appeared to result from charge state coalescence.
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影响因子：
2.3
作者：
LOO, RRO;SMITH, RD
通讯作者：
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影响因子：
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DOI：
10.1007/s13361-011-0319-1
发表时间：
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影响因子：
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通讯作者：
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作者：
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