Topography and morphometry of capillaries in the rat subfornical organ.
Topography and morphometry of capillaries in the rat subfornical organ.
复制标题
大鼠穹窿下器官毛细血管的地形和形态测量。
DOI：
10.1002/cne.902600104
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Gross,PM
中科院分区：
文献类型：
--
作者：
Sposito,NM;Gross,PM
对Long-Evans、纯合子Brattleboro和Spraogue-Dawley大鼠进行了全面的体视学分析，以确定穹隆下器官个别亚区的毛细血管尺寸。在矢状面的四个区域(吻侧、移行、中央和尾侧)和冠状平面的两个区域(背侧和腹内侧区)评估毛细血管密度、体积分数、长度、表面积和直径。中央和尾侧的腹内侧区对应于穹隆下器的致密核周区和含有神经传入的神经束(例如，推测为血管紧张素II的纤维终末)，而这些区域的背侧区显然是核周区的优势部位，其传出投射直接指向器官外。形态计量学分析显示，穹隆下器各亚区的毛细血管密度不均(三组大鼠的范围为132至931根毛细血管/毫米~2)。中央和尾侧腹内侧区的毛细血管密度、体积分数和表面积显著高于邻近背侧区和吻侧区，但直径较小。在背侧带内的所有亚区，三组大鼠的形态计量学模式大体一致。通过对Long-Evans大鼠穹隆下器官各亚区内皮细胞的定性电子显微镜观察，发现至少有三种类型的毛细血管按区域定向：(1)在吻部，毛细血管无内皮窗或毛细血管周围间隙，极少的小泡；(2)在吻部和中央区的“过渡区”，毛细血管无内皮细胞窗口，有大量的小泡，狭窄但可察觉的尖周间隙；(3)在中央和尾部。毛细血管具有丰富的内皮窗口和小泡，扩张的毛细血管周围迷路和相对较薄的壁。从光镜形态计量学和电子显微镜的这些发现表明，大鼠的毛细血管组织具有异质性，这与细胞学和推测的穹隆下器官的功能相对应。
A comprehensive stereological analysis was performed to define capillary dimensions in individual subregions of the subfornical organ in Long‐Evans, homozygous Brattleboro, and Sprague‐Dawley rats. Capillary density, volume fraction, length, surface area, and diameter were assessed in four regions in the sagittal plane (rostral, “transitional,” central, and caudal) and two zones in the coronal plane (dorsal and ventromedial). The ventromedial zones in the central and caudal regions correspond to areas of dense perikarya and neuropil containing neural afferent inputs to the subfornical organ (e.g., putative fiber terminals for angiotensin II), whereas the dorsal zones of these regions are apparently the predominant sites of perikarya having efferent projections directed outside of the organThe morphometric analysis revealed heterogeneous capillary density across subregions of the subfornical organ (range of 132 to 931 capillaries/mm2in the three rat groups). Capillaries in the ventromedial zones of the central and caudal regions had significantly greater density, volume fraction, and surface area, but smaller diameters, than those in the adjacent dorsal zones and more rostral regions. Across all subregions within the dorsal zone, there was generally a consistent morphometric pattern in the three rat groups. No differences in capillary dimensions in any part of the subfornical organ were found between the Long‐Evans and Brattleboro rats.A qualitative electron microscopic investigation of endothelial cells in each subregion of the subfornical organ in Long‐Evans rats revealed at least three types of capillary oriented according to region: (1) in the rostral region were capillaries having no endothelial fenestrations or pericapillary spaces, and few vesicles, (2) in the “transitional” region between the rostral and central regions, capillaries having no endothelial fenestrations, substantial numbers of vesicles, and narrow but perceptible pericapillary spaces were found, and (3) in the central and caudal regions, capillaries having abundant endothelial fenestrations and vesicles, expansive pericapillary labyrinths, and relatively thin walls were present.These findings from light microscopic morphometry and electron microscopy in rats indicate a heterogeneity of capillary organization that shows topographical correspondence to the cytology and putative functions of the subfornical organ.