Mechanism of DNA cleavage by the DNA/RNA-non-specific Anabaena sp PCC 7120 endonuclease NucA and its inhibition by NuiA
Mechanism of DNA cleavage by the DNA/RNA-non-specific Anabaena sp PCC 7120 endonuclease NucA and its inhibition by NuiA
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DOI：
10.1006/jmbi.2000.3586
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发表时间：
2000-03-24
影响因子：
5.6
通讯作者：
Pingoud, A
中科院分区：
文献类型：
--
作者：
Meiss, G;Gimadutdinow, O;Pingoud, A
基于相关沙雷氏菌核酸酶的三维结构获得的来自鱼腥藻PCC 7120的DNA/RNA非特异性核酸内切酶NucA的结构模型表明，包括氨基酸残基H124、N155和E163（对应于沙雷氏菌核酸酶中的H89、N119和E127）的活性位点的总体结构在两种核酸酶中相似。这些残基被丙氨酸取代导致活性的大幅降低（
A structural model of the DNA/RNA non-specific endonuclease NucA from Anabaena sp. PCC7120 that has been obtained on the basis of the three-dimensional structure of the related Serratia nuclease, suggests that the overall architecture of the active site including amino acid residues H124, N155 and E163 (corresponding to H89, N119 and E127 in Serratia nuclease) is similar in both nucleases. Substitution of these residues by alanine leads to a large reduction In activity (