COMPLEMENT FIXATION TECHNIQUE FOR QUANTITATIVE MEASUREMENT OF ANTIGENIC DIFFERENCES BETWEEN STRAINS OF VIRUS OF FOOT-+-MOUTH DISEASE
COMPLEMENT FIXATION TECHNIQUE FOR QUANTITATIVE MEASUREMENT OF ANTIGENIC DIFFERENCES BETWEEN STRAINS OF VIRUS OF FOOT-+-MOUTH DISEASE
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DOI：
10.1017/s0022172400040146
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发表时间：
1964-01-01
影响因子：
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通讯作者：
DAVIE, J
中科院分区：
文献类型：
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作者：
DAVIE, J
已经开发了一种“补体结合试验”，用于测量相同免疫类型的口蹄疫病毒株之间的微小抗原差异。从检查的每种病毒株制备菌株特异性血清，然后用每种抗原滴定。在抗原和抗体以最佳比例存在的最大固定区域测量每个反应中固定的补体量。在每项试验中，将异源抗原-抗体反应与同源反应进行比较，并表示为交叉固定率。然后可以通过将它们的交叉固定比率的乘积与另一个进行比较抗原对。由此获得的交叉固定产物的值范围从相同菌株之间的1.0到不同免疫型菌株之间的0.01。显示出相互交叉固定产物为0.5或更高的病毒株已被分类在亚型组内。
A " complement fixation test has been developed to measure the minor antigenic differences which occur between strains of the virus of foot-and-mouth disease of the same immunological type. Strain-specific sera were prepared from each of the virus strains examined and then titrated with each of the antigens. The amounts of complement fixed in each reaction were measured in the region of maximal fixation where the antigen and antibody were present in optimal proportions. Heterologous antigen-antibody reactions were compared with the homologous reaction in each test and expressed as cross-fixation ratios. Pairs of antigens could then be compared by taking the product of their cross-fixation ratios one with the other. The values of cross-fixation product so obtained ranged from 1.0 between identical strains to 0.01 between strains of different immunological type. Strains of virus which exhibit cross-fixation products of 0.5 or more with one another have been classified within subtype groups.