Hemodynamic disturbances in the rat as a function of the number of microspheres injected.
Hemodynamic disturbances in the rat as a function of the number of microspheres injected.
复制标题
大鼠的血流动力学紊乱与注射的微球数量有关。
DOI：
10.1152/ajpheart.1983.245.6.h920
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Coleman,TG
中科院分区：
文献类型：
--
作者：
Stanek,KA;Smith,TL;Murphy,WR;Coleman,TG
本研究的目的是重新评估用于测量血流分布的放射性微球技术。老鼠在研究时是有意识的。使用比重为1.3的葡萄糖溶液作为悬浮介质，而不是10%葡聚糖，后者先前已显示会引起低血压。将微球注入左心房，在注入主动脉弓之前与血液进行最大程度的混合。在每个参考样品后给予Ficoll-70作为液体置换。通过这些修改，注射360，000个微球或更少的微球不会引起血液动力学紊乱，如通过电磁流量计所判断的。在大鼠体内累积了1.4 × 10（6）个微球（多次注射）后，唯一显著的血流动力学紊乱是心率降低。本研究确定了大鼠中在血流动力学紊乱发生前可注射的微球数量的限度。
The purpose of this study was to reevaluate the radioactive microsphere technique used to measure blood flow distribution. The rats were conscious when studied. A dextrose solution with specific gravity of 1.3 was used as the suspension media instead of 10% dextran, which has previously been shown to cause hypotension. The microspheres were injected into the left atrium, which provided for maximal mixing with the blood before being ejected into the aortic arch. Ficoll-70 was given after each reference sample as a fluid replacement. With these modifications an injection of 360,000 microspheres or less caused no hemodynamic disturbances, as judged by electromagnetic flowmetry. After 1.4 X 10(6) microspheres had accumulated in the rat (several injections) the only significant hemodynamic disturbance was a decreased heart rate. This study establishes the limits in the rat regarding the number of microspheres that can be injected before hemodynamic disturbances result.