The characteristics of peptide collision-induced dissociation using a high-performance MALDI-TOF/TOF tandem mass spectrometer
The characteristics of peptide collision-induced dissociation using a high-performance MALDI-TOF/TOF tandem mass spectrometer
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DOI：
10.1021/ac990809y
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发表时间：
2000-02-01
影响因子：
7.4
通讯作者：
Burlingame, AL
中科院分区：
文献类型：
--
作者：
Medzihradszky, KF;Campbell, JM;Burlingame, AL
描述了一种新型的基质辅助激光解吸/电离（MALDI）飞行时间/飞行时间（TOF/TOF）高分辨率串联质谱仪，该仪器结合了MALDI对肽分析的高灵敏度和高能碰撞诱导解离（CID）提供的全面碎片化信息的优点。与MALDI-TOF仪器广泛使用的后源衰减技术不同，该仪器通常结合多达10个不同质量区域的独立光谱，该仪器允许在固定反射电压下的单次采集中获得完整的碎片离子光谱。为了在整个质量范围内获得最佳分辨率和聚焦，可能需要获取并组合三个单独的部分。MALDI基质和碰撞气体的不同组合决定了MALDI过程和CID过程沉积的内能量，从而控制了观察到的碎片离子的程度和性质。以肽段测序为例，对序列相关特征进行了识别，并证明了修改电离和碰撞条件以优化光谱信息的价值。
A new matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) high-resolution tandem mass spectrometer is described for sequencing peptides, This instrument combines the advantages of high sensitivity for peptide analysis associated with MALDI and comprehensive fragmentation information provided by high-energy collision-induced dissociation (CID). Unlike the postsource decay technique that is widely used with MALDI-TOF instruments and typically combines as many as 10 separate spectra of different mass regions, this instrument allows complete fragment ion spectra to be obtained in a single acquisition at a fixed reflection voltage. To achieve optimum resolution and focusing over the whole mass range, it may be desirable to acquire and combine three separate sections. Different combinations of MALDI matrix and collision gas determine the amount of internal energy deposited by the MALDI process and the CID process, which provide control over the extent and nature of the fragment ions observed. Examples of peptide sequencing are presented that identify sequence-dependent features and demonstrate the value of modifying the ionization and collision conditions to optimize the spectral information.