Normal adult climbing fiber monoinnervation of cerebellar Purkinje cells in mice lacking MHC class I molecules
Normal adult climbing fiber monoinnervation of cerebellar Purkinje cells in mice lacking MHC class I molecules
复制标题
DOI：
10.1002/dneu.20639
复制
发表时间：
2008-07-01
影响因子：
3
通讯作者：
Lohof, Ann M.
中科院分区：
文献类型：
--
作者：
Letellier, Mathieu;Willson, Melina L.;Lohof, Ann M.
最近，一些免疫系统蛋白质与神经元连接的发育和可塑性有关。值得注意的是，主要组织相容性复合体1（MHC 1类）的蛋白质已被证明参与海马中的突触可塑性和视觉系统中投射模式的发展。我们研究了MHC 1类蛋白在一个典型的突触繁荣和随后的细化的例子中可能发挥的作用，即攀缘纤维（CF）到浦肯野细胞（PC）突触。对缺乏两种MHC基因H2-D1和H2-K1以及β 2-微球蛋白基因的成年小鼠的小脑进行了检查，以寻找其PC上多余CF突触消除不足的证据。电生理和形态学证据表明，尽管没有这些MHC 1类分子，成年PC在这些转基因小鼠是单神经支配的野生型动物。这些研究结果表明，在这个突触的传入数量的限制水平，功能性MHC 1类蛋白是不需要的。(c)2008 Wiley Periodicals，Inc.
Some immune system proteins have recently been implicated in the development and plasticity of neuronal connections. Notably, proteins of the major histocompatibility complex 1 (MHC class 1) have been shown to be involved in synaptic plasticity in the hippocampus and the development of projection patterns in the visual system. We examined the possible role for the MHC class 1 proteins in one well-characterized example of synaptic exuberance and subsequent refinement, the climbing fiber (CF) to Purkinje cell (PC) synapse. Cerebella from adult mice deficient for two MHC genes, H2-D1 and H2-K1, and for beta 2-microglobulin gene were examined for evidence of deficient elimination of supernumerary CF synapses on their PCs. Electrophysiological and morphological evidence showed that, despite the absence of these MHC class 1 molecules, adult PCs in these transgenic mice are monoinnervated as in wild-type animals. These findings indicate that, at the level of restriction of afferent number at this synapse, functional MHC class 1 proteins are not required. (c) 2008 Wiley Periodicals, Inc.