Phosphatidylinositol 4-kinaseβ is critical for functional association of rab11 with the Golgi complex
Phosphatidylinositol 4-kinaseβ is critical for functional association of rab11 with the Golgi complex
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DOI：
10.1091/mbc.e03-12-0862
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发表时间：
2004-04-01
影响因子：
3.3
通讯作者：
Henegouwen, PMPVE
中科院分区：
文献类型：
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作者：
de Graaf, P;Zwart, WT;Henegouwen, PMPVE
磷脂酰肌醇4-激酶β (pi4kβ)在维持高尔基复合体的结构完整性中起着重要作用。在寻找PI4Kbeta相互作用蛋白的过程中，我们发现PI4Kbeta特异性地与gtp结合形式的小GTPase rab11相互作用。pi4kβ -rab11的相互作用具有重要的功能意义，因为抑制rab11与pi4kβ的结合消除了rab11在高尔基复合体上的定位，并显著抑制了水泡性口炎病毒G蛋白从高尔基复合体向质膜的运输。我们提出pi4kβ的一个新功能是作为高尔基复合体中rab11的对接蛋白，这对于从高尔基复合体到质膜的生物合成膜运输是重要的。
Phosphatidylinositol 4-kinasebeta (PI4Kbeta) plays an essential role in maintaining the structural integrity of the Golgi complex. In a search for PI4Kbeta-interacting proteins, we found that PI4Kbeta specifically interacts with the GTP-bound form of the small GTPase rab11. The PI4Kbeta-rab11 interaction is of functional significance because inhibition of rab11 binding to PI4Kbeta abolished the localization of rab11 to the Golgi complex and significantly inhibited transport of vesicular stomatitis virus G protein from the Golgi complex to the plasma membrane. We propose that a novel function of PI4Kbeta is to act as a docking protein for rab11 in the Golgi complex, which is important for biosynthetic membrane transport from the Golgi complex to the plasma membrane.