Identification of mitogen-activated protein kinase kinase gene family and MKK-MAPK interaction network in maize
Identification of mitogen-activated protein kinase kinase gene family and MKK-MAPK interaction network in maize
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DOI：
10.1016/j.bbrc.2013.11.008
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发表时间：
2013-11-29
影响因子：
3.1
通讯作者：
Li, Dequan
中科院分区：
文献类型：
--
作者：
Kong, Xiangpei;Pan, Jiaowen;Li, Dequan
植物丝裂原激活蛋白激酶 (MAPK) 参与重要过程，包括应激信号传导和发育。 MAPK 激酶（MAPKK、MKK）已在多种植物物种中进行了研究，包括拟南芥、水稻、毛果杨和二穗短柄草。在本研究中，已鉴定出九个假定的玉米 MKK 基因。对保守蛋白基序、外显子-内含子连接和内含子阶段的分析揭示了系统发育群体内的高水平保守性。接下来，我们使用酵母双杂交定义了四种新的 ZmMKK-ZmMPK 相互作用。最后，我们检查了 ZmMKK4 基因的生物学功能。与野生型植物相比，拟南芥中 ZmMKK4 的过表达通过提高发芽率和早期幼苗生长而赋予氧化应激耐受性。总之，我们对玉米基因组中的 MKK 基因家族进行了全面的生物信息学分析，我们的数据为玉米中 MAPK 和 MKK 家族的进一步功能研究提供了重要基础。 (C) 2013 Elsevier Inc. 保留所有权利。
Plant mitogen-activated protein kinases (MAPK) are involved in important processes, including stress signaling and development. MAPK kinases (MAPKK, MKK) have been investigated in several plant species including Arabidopsis thaliana, Oryza sativa, Populus trichocarpa, and Brachypodium distachyon. In the present study, nine putative maize MKK genes have been identified. Analysis of the conserved protein motifs, exon-intron junctions and intron phase has revealed high levels of conservation within the phylogenetic groups. Next, we defined four new ZmMKK-ZmMPK interactions using yeast two-hybrid. Finally, we examined the biological functions of the ZmMKK4 gene. Overexpression of ZmMKK4 in Arabidopsis conferred tolerance to oxidative stress by increased germination rate and early seedling growth compared with WT plants. Taken together, we provide a comprehensive bioinformatics analysis of the MKK gene family in maize genome and our data provide an important foundation for further functional study of MAPK and MKK families in maize. (C) 2013 Elsevier Inc. All rights reserved.