Exopolysaccharides produced by Burkholderia cenocepacia recA lineages IIIA and IIIB.
Exopolysaccharides produced by Burkholderia cenocepacia recA lineages IIIA and IIIB.
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DOI：
10.1016/j.jcf.2004.04.004
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发表时间：
2004-08-01
期刊：
影响因子：
--
通讯作者：
Rizzo, Roberto
中科院分区：
文献类型：
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作者：
Chiarini, Luigi;Cescutti, Paola;Rizzo, Roberto
检查了属于recA谱系IIIA和IIIB的新洋葱伯克霍尔德氏菌的临床和环境菌株的胞外多糖（EPS）生产。主要使用气相色谱法结合质谱法和NMR光谱法测定胞外多糖的结构。所有菌株产生Cepacian，一种高度分支的多糖，由七糖重复单元组成，由一个鼠李糖、一个葡萄糖、一个葡萄糖醛酸、一个甘露糖和三个半乳糖残基组成。这种聚合物是洋葱伯克霍尔德菌（Bcc）复合体菌株产生的最常见的外多糖。一种临床菌株还产生另一种由三个半乳糖单元和一个3-脱氧-D-甘露-2-辛酮糖酸残基组成的多糖，该聚合物先前从B的两种菌株中分离。洋葱基因型I和B。新洋葱属
Clinical and environmental strains of Burkholderia cenocepacia belonging to the recA lineages IIIA and IIIB were examined for exopolysaccharide (EPS) production. The exopolysaccharides structure was determined using mainly gas chromatography coupled to mass spectrometry and NMR spectroscopy. All the strains produced Cepacian, a highly branched polysaccharide constituted of a heptasaccharide repeating unit, composed of one rhamnose, one glucose, one glucuronic acid, one mannose and three galactose residues. This polymer is the most common exopolysaccharide produced by strains of the Burkholderia cepacia (Bcc) complex. One clinical strain produced also another polysaccharide constituted of three galactose units and one 3-deoxy-D-manno-2-octulosonic acid residues, a polymer that was previously isolated from two strains of B. cepacia genomovar I and B. cenocepacia IIIA.