Improved HPLC method for the determination of curcumin, demethoxycurcumin, and bisdemethoxycurcumin
Improved HPLC method for the determination of curcumin, demethoxycurcumin, and bisdemethoxycurcumin
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DOI：
10.1021/jf025506a
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发表时间：
2002-06-19
影响因子：
6.1
通讯作者：
Sakariah, KK
中科院分区：
文献类型：
--
作者：
Jayaprakasha, GK;Rao, LJM;Sakariah, KK
市售的姜黄素是一种明亮的橙黄色姜黄色素，由三种姜黄素类化合物即姜黄素、脱甲氧基姜黄素和双脱甲氧基姜黄素的混合物组成。通过柱色谱法分离这些化合物，并通过光谱研究鉴定。用改进的HPLC法分析姜黄素类化合物的纯度。采用C-18柱，使用甲醇、2%AcOH和乙腈三种溶剂进行HPLC分离，检测波长为425 nm。分析了四种不同的市售姜黄品种，即Salem、Erode、巴拉索雷和当地市场样品，以检测这三种姜黄素的百分比。发现使用其校准曲线估计的姜黄素、脱甲氧基姜黄素和双脱甲氧基姜黄素的百分比分别为1.06 +/- 0.061至5.65 +/- 0.040、0.83 +/- 0.047至3.36 +/- 0.040和0.42 +/- 0.036至2.16 +/- 0.06，在四个不同的样本中。姜黄素类化合物的总百分比为2.34 +/- 0.171至9.18 +/-0.232%。
Commercially available curcumin, a bright orange-yellow color pigment of turmeric, consists of a mixture of three curcuminoids, namely, curcumin, demethoxycurcumin, and bisdemethoxycurcumin. These were isolated by column chromatography and identified by spectroscopic studies. The purity of the curcuminoids was analyzed by an improved HPLC method. HPLC separation was performed on a C-18 column using three solvents, methanol, 2% AcOH, and acetonitrile, with detection at 425 nm. Four different commercially available varieties of turmeric, namely, Salem, Erode, Balasore, and local market samples, were analyzed to detect the percentage of these three curcuminoids. The percentages of curcumin, demethoxycurcumin, and bisdemethoxycurcumin as estimated using their calibration curves were found to be 1.06 +/- 0.061 to 5.65 +/- 0.040, 0.83 +/- 0.047 to 3.36 +/- 0.040, and 0.42 +/- 0.036 to 2.16 +/- 0.06, respectively, in four different samples. The total percentages of curcuminoids are 2.34 +/- 0.171 to 9.18 +/- 0.232%.