Control of Grain Size and Weight by the OsMKKK10-OsMKK4-OsMAPK6 Signaling Pathway in Rice
Control of Grain Size and Weight by the OsMKKK10-OsMKK4-OsMAPK6 Signaling Pathway in Rice
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OsMKKK10-OsMKK4-OsMAPK6 信号通路对水稻籽粒大小和重量的控制
DOI：
10.1016/j.molp.2018.04.004
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发表时间：
2018
期刊：
影响因子：
27.5
通讯作者：
Li Yunhai
中科院分区：
文献类型：
--
作者：
Xu Ran;Duan Penggen;Yu Haiyue;Zhou Zhengkui;Zhang Baolan;Wang Ruci;Li Jing;Zhang Guozheng;Zhuang Shangshang;Lyu Jia;Li Na;Chai Tuanyao;Tian Zhixi;Yao Shanguo;Li Yunhai
籽粒大小是决定作物产量的关键农艺性状之一。然而，农作物中的粒度控制机制仍然难以捉摸。在此，我们证明了OsMKKK 10-OsMKK 4-OsMAPK 6信号通路正调控水稻的籽粒大小和重量。在水稻中，OsMKKK 10功能的缺失导致小而轻的籽粒、短穗和半矮秆植株，而组成型活性OsMKK 10（CA-OsMKK 10）的过表达导致大而重的籽粒、长穗和高植株。OsMKKK 10与OsMKK 4相互作用并使其磷酸化。我们鉴定了产生大且重的谷粒的0 sMKK 4功能获得性突变体（large 11 -1D）。OsMKK 4 A227 T由大11 - 1D等位基因编码，具有比OsMKK 4更强的激酶活性。过表达OsMKK 4的组成型活性形式（OsMKK 4-DD）的植物也产生大谷粒。进一步的生化和遗传分析表明，OsMKKK 10，OsMKK 4和OsMAPK 6在一个共同的途径控制晶粒大小的功能。综上所述，我们的研究为OsMKKK 10-OsMKK 4-OsMAPK 6级联调控水稻籽粒大小和重量建立了重要的遗传和分子框架。
Grain size is one of the key agronomic traits that determine grain yield in crops. However, the mechanisms underlying grain size control in crops remain elusive. Here we demonstrate that the OsMKKK10-OsMKK4-OsMAPK6 signaling pathway positively regulates grain size and weight in rice. In rice, loss ofOsMKKK10function results in small and light grains, short panicles, and semi-dwarf plants, while overexpression of constitutively active OsMKKK10 (CA-OsMKKK10) results in large and heavy grains, long panicles, and tall plants. OsMKKK10 interacts with and phosphorylates OsMKK4. We identified anOsMKK4gain-of-function mutant (large11-1D) that produces large and heavy grains. OsMKK4A227Tencoded by thelarge11-1Dallele has stronger kinase activity than OsMKK4. Plants overexpressing a constitutively active form of OsMKK4 (OsMKK4-DD) also produce large grains. Further biochemical and genetic analyses revealed that OsMKKK10, OsMKK4, and OsMAPK6 function in a common pathway to control grain size. Taken together, our study establishes an important genetic and molecular framework for OsMKKK10-OsMKK4-OsMAPK6 cascade-mediated control of grain size and weight in rice.