Investigation of the role of healthy dogs as potential carriers of rabies virus
Investigation of the role of healthy dogs as potential carriers of rabies virus
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DOI：
10.1089/vbz.2007.0209
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发表时间：
2008-06-01
影响因子：
2.1
通讯作者：
Rupprecht, Charles E.
中科院分区：
文献类型：
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作者：
Zhang, Yong-Zhen;Fu, Zhen F.;Rupprecht, Charles E.
为了解健康动物是否为中国狂犬病病毒的潜在携带者，在贵州省安龙县狂犬病疫区收集了153只家犬，并进行了6个月的监测。最初，通过酶联免疫吸附试验（ELISA）在15只狗的唾液中发现狂犬病毒抗原，表明它们可能是携带者。对这15只狗进行了6个月的观察。在观察期间，没有一只狗表现出狂犬病的临床症状。此外，单独使用ELISA检测，观察期间部分动物唾液中狂犬病毒抗原的检测结果不一致。然而，通过逆转录-聚合酶链反应（RT-PCR），在采集时或观察期间收集的唾液样本均未发现狂犬病毒RNA阳性。此外，在安乐死时收集的大脑样本中既没有检测到病毒抗原，也没有检测到病毒RNA。这些结果不支持健康犬是狂犬病携带者的论点。当使用初步和不确定的测试（如ELISA）来推断与狂犬病有关的临床状态时，应谨慎行事。
To investigate whether healthy animals are potential carriers of rabies virus in China, 153 domestic dogs were collected from a rabies enzootic area, Anlong county in Guizhou Province, and monitored for 6 months. Initially, findings of rabies virus antigen in the saliva of 15 dogs by an enzyme-linked immunosorbent assay (ELISA) test suggested they might be carriers. These 15 dogs were kept under observation for 6 months. None of the dogs showed any clinical signs of rabies during the observation period. Moreover, using the ELISA test alone, detection of rabies virus antigen in saliva of some animals was not consistent during the observation period. However, none of the saliva samples collected either at the time of acquisition or during the observation period was found to be positive for rabies virus RNA by reverse transcriptase-polymerase chain reaction (RT-PCR). Furthermore, neither viral antigen nor viral RNA was detected in the brain samples collected at the time of euthanasia. These results do not provide support for the contention that healthy dogs act as carriers in rabies. Caution is urged when preliminary and nondefinitive tests, such as ELISA, are used to infer clinical status related to rabies.