Combined PET/DCE-MRI in a Rabbit Model of Atherosclerosis
Combined PET/DCE-MRI in a Rabbit Model of Atherosclerosis
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DOI：
10.1016/j.jcmg.2017.11.030
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发表时间：
2018-02-01
影响因子：
14
通讯作者：
Fayad, Zahi A.
中科院分区：
文献类型：
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作者：
Calcagno, Claudia;Lairez, Olivier;Fayad, Zahi A.
作者试图开发组合的正电子发射断层扫描（PET）动态对比增强（DCE）磁共振成像（MRI），以量化斑块炎症、渗透性和负荷，从而评估白三烯A4水解酶（LTA 4 H）抑制剂在兔动脉粥样硬化模型中的功效。通过组合的F-18-氟脱氧葡萄糖（F-18-FDG）PET、DCE-MRI和形态MRI测定负荷。作者描述了一种新的，综合PET-DCE/MRI协议，以非侵入性量化这些参数的兔动脉粥样硬化模型的主动脉斑块。作为概念验证，作者应用该方案评估新型LTA 4 H抑制剂BI 691751的疗效。方法新西兰白色雄性家兔（N = 49）在动脉粥样硬化诱导后和随机分为3组后1个月和3个月进行PET-DCE/MRI成像：1）安慰剂组; 2）高剂量BI 691751组; 3）低剂量BI 691751组。结果在几个量化的指标中，只有最大标准化摄取值和F-18-FDG PET的目标与背景比显示出用低剂量BI 691751治疗的兔中斑块炎症的适度但显著的减少（p = 0.03），而在高脂肪饮食和高剂量BI 691751组中未检测到差异。在任何组中均未检测到MRI测量的血管壁面积和DCE-MRI测量的曲线下面积存在差异。在neovessel和巨噬细胞密度没有差异，发现在研究结束时groups.CONCLUSIONS作者提出了一个全面的，综合的F-18-FDG PET和DCE-MRI成像协议，以非侵入性量化斑块炎症，新生血管，渗透性和负担在兔模型动脉粥样硬化的同时PET/MRI扫描仪。在用低剂量LTA 4 H抑制剂BI 691751治疗的组中，通过F-18-FDG PET发现斑块炎症适度减少。(C)2018年由美国心脏病学会基金会。
OBJECTIVES The authors sought to develop combined positron emission tomography (PET) dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) to quantify plaque inflammation, permeability, and burden to evaluate the efficacy of a leukotriene A4 hydrolase (LTA4H) inhibitor in a rabbit model of atherosclerosis.BACKGROUND Multimodality PET/MRI allows combining the quantification of atherosclerotic plaque inflammation, neovascularization, permeability, and burden by combined F-18-fluorodeoxyglucose (F-18-FDG) PET, DCE-MRI, and morphological MRI. The authors describe a novel, integrated PET-DCE/MRI protocol to noninvasively quantify these parameters in aortic plaques of a rabbit model of atherosclerosis. As proof-of-concept, the authors apply this protocol to assess the efficacy of the novel LTA4H inhibitor BI691751.METHODS New Zealand White male rabbits (N = 49) were imaged with integrated PET-DCE/MRI after atherosclerosis induction and 1 and 3 months after randomization into 3 groups: 1) placebo; 2) high-dose BI691751; and 3) low-dose BI691751. All animals were euthanized at the end of the study.RESULTS Among the several metrics that were quantified, only maximum standardized uptake value and target-tobackground ratio by F-18-FDG PET showed a modest, but significant, reduction in plaque inflammation in rabbits treated with low-dose BI691751 (p = 0.03), whereas no difference was detected in the high-fat diet and in the high-dose BI691751 groups. No differences in vessel wall area by MRI and area under the curve by DCE-MRI were detected in any of the groups. No differences in neovessel and macrophage density were found at the end of study among groups.CONCLUSIONS The authors present a comprehensive, integrated F-18-FDG PET and DCE-MRI imaging protocol to noninvasively quantify plaque inflammation, neovasculature, permeability, and burden in a rabbit model of atherosclerosis on a simultaneous PET/MRI scanner. A modest reduction was found in plaque inflammation by F-18-FDG PET in the group treated with a low dose of the LTA4H inhibitor BI691751. (C) 2018 by the American College of Cardiology Foundation.