Tyrosine kinase signaling involves in glutamate-induced astrocyte proliferation
Tyrosine kinase signaling involves in glutamate-induced astrocyte proliferation
复制标题
酪氨酸激酶信号传导参与谷氨酸诱导的星形胶质细胞增殖
作者：
Su;Chun
星形胶质细胞的增殖是CNS对损伤和疾病的常见反应。控制星形胶质细胞增殖的机制是非常感兴趣的。在本文中，谷氨酸诱导的星形胶质细胞增殖的信号通路进行了研究。谷氨酸刺激大鼠皮层星形胶质细胞非同步、亚融合培养物的增殖。G蛋白、蛋白激酶A、蛋白激酶C、磷脂酰肌醇3激酶、细胞外信号调节激酶或磷脂酶A2的抑制剂不能阻止谷氨酸诱导的细胞增殖。酪氨酸激酶抑制剂Genistein和Herbimycin A抑制谷氨酸诱导的细胞增殖。此外，这种增殖是由谷氨酸代谢型受体的激活介导的。这些结果表明，谷氨酸通过酪氨酸激酶途径诱导星形胶质细胞增殖。
Proliferation of astrocytes is a common response of the CNS to injury and disease. The mechanisms controlling the proliferation of astrocytes are of great interest. In this paper, the signaling pathways underlying glutamate-induced astrocyte proliferation are investigated. Glutamate stimulates the proliferation of non-synchronized, subconfluent cultures of rat cortical astrocytes. Glutamate-induced cell proliferation is not prevented by inhibitors of G protein, protein kinase A, protein kinase C, phosphatidylinositol 3 kinase, extracellular signal-regulated kinase, or phospholipase A2. However, the tyrosine kinase inhibitors Genistein and Herbimycin A inhibit the glutamate-induced proliferation. Moreover, this proliferation is mediated by the activation of glutamate metabotropic receptors. These results suggest that glutamate induces astrocyte proliferation through a tyrosine kinase pathway.