Role of molecular charge in glomerular permeability. Tracer studies with cationized ferritins.
Role of molecular charge in glomerular permeability. Tracer studies with cationized ferritins.
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DOI：
10.1083/jcb.67.3.638
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发表时间：
1975-12
期刊：
影响因子：
--
通讯作者：
Venkatachalam MA
中科院分区：
文献类型：
--
作者：
Rennke HG;Cotran RS;Venkatachalam MA
用Krebs-Ringer碳酸氢盐缓冲液(KRB)灌流小鼠肾脏，KRB含有天然的、阴离子的马脾铁蛋白或各种阳离子衍生物，通过电子显微镜观察探针分子在肾小球的定位。相对于介质(KRB，pH 7.45)的阳离子铁素在肾小球基底膜(GBM)内皮下层累积的量远远超过阴离子铁素，阳离子程度越高的衍生物越严重。此外，强阳离子铁蛋白还大量渗透到基底膜的整个厚度，但在滤过缝隙的水平上似乎阻碍了进入尿腔的速度。非常强阳离子的衍生物附着于肾小球内皮细胞和基底膜，并在后者的外层形成聚集体。结果提示，肾小球基底膜和血管内皮细胞存在内源性负电荷，肾小球毛细血管壁的屏障功能可能部分归因于其电物理性质。
Mouse kidneys were perfused with Krebs-Ringer bicarbonate buffer (KRB) containing native, anionic horse spleen ferritin or various cationized derivatives, and the glomerular localization of the probe molecules determined by electron microscopy. Ferritins cationic with respect to the medium (KRB, pH 7.45) accumulated in the subendothelial layers of the glomerular basement membrane (GBM) in amounts far exceeding those observed with anionic ferritins, the degree being greater for the more cationized derivatives. Strongly cationized ferritins, in addition permeated the full thickness of the GBM in considerable amounts, but appeared to be retarded from entry into the urinary spaces at the level of the filtration slits. Very strongly cationized derivatives adhered to glomerular endothelium and GBM and formed aggregates in the outer layers of the latter. The results suggest that intrinsic negative charges are present in the GBM and endothelium, and that the barrier function of the glomerular capillary wall may be ascribed in part to its electrophysical properties.