Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish
Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish
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DOI：
10.1016/s0145-305x(03)00036-3
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发表时间：
2003-06-01
影响因子：
2.9
通讯作者：
Tsoi, SCM
中科院分区：
文献类型：
--
作者：
Douglas, SE;Gallant, JW;Tsoi, SCM
抗菌肽作为抵御病原体入侵的第一道防线发挥着至关重要的作用。已从鱼类中分离出几种类型的抗菌肽，其中大多数是阳离子α-螺旋品种。在这里，我们展示了来自冬鲽鱼 Pseudopleuronectes americanus (Walbaum) 的 5 种高度二硫键键合的铁调素样肽和两种来自大西洋鲑鱼 Salmo salar (L.) 的 cDNA 序列。除了 19-27 个氨基酸的成熟加工肽外，这些铁调素样分子还包含 24 个氨基酸的信号肽和 38-40 个氨基酸的酸性前体。对 GenBank 对这些序列的详尽数据挖掘表明，日本比目鱼、虹鳟鱼、杂交条纹鲈鱼和青鳉的基因组中编码了类似的肽，表明它们在鱼类中广泛存在。 Southern杂交分析表明，密切相关的铁调素样基因也存在于其他比目鱼物种中，并且它们作为聚集在冬鲽基因组上的多基因家族存在。铁调素变体在细菌攻击期间、美洲对虾幼体发育期间以及成鱼的不同组织中表达有差异。 (C) 2003 年，爱思唯尔科学有限公司出版。
Antimicrobial peptides play a crucial role as the first line of defense against invading pathogens. Several types of antimicrobial peptides have been isolated from fish, mostly of the cationic alpha-helical variety. Here, we present the cDNA sequences of five highly disulphide-bonded hepcidin-like peptides from winter flounder, Pseudopleuronectes americanus (Walbaum) and two from Atlantic salmon, Salmo salar (L.). These hepcidin-like molecules consist of a 24 amino acid signal peptide and an acidic propiece of 38-40 amino acids in addition to the mature processed peptide of 19-27 amino acids. Exhaustive data mining of GenBank with these sequences revealed that similar peptides are encoded in the genomes of Japanese flounder, rainbow trout, hybrid striped bass and medaka, indicating that they are widespread among fish. Southern hybridization analysis suggests that closely related hepcidin-like genes are present in other flatfish species, and that they exist as a multigene family clustered on the winter flounder genome. Hepcidin variants are differentially expressed during bacterial challenge, during larval development of P. americanus and in different tissues of adult fish. (C) 2003 Published by Elsevier Science Ltd.