A sensitive method for determining levels of [-]-2,5,-dimethoxy-4-methylamphetamine in the brain tissue
A sensitive method for determining levels of [-]-2,5,-dimethoxy-4-methylamphetamine in the brain tissue
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DOI：
10.1016/s1056-8719(02)00159-4
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发表时间：
2001-07-01
影响因子：
1.9
通讯作者：
Winter, JC
中科院分区：
文献类型：
--
作者：
Eckler, JR;Greizerstein, H;Winter, JC
简介：吲哚胺和苯乙胺致幻剂是滥用药物，以及模仿特发性精神病的某些方面。（-）2,5-二甲氧基-4-甲基安非他明（[-]- dom）是5-羟色胺类苯乙胺致幻剂中的一员，为了协助研究其作用机制，需要一种灵敏而精确的方法来测定其在脑组织中的水平。方法：我们现在描述了一种测定大鼠脑组织中[-]- dom纳克量的方法，使用d -安非他明作为内标。该方法采用甲苯溶剂萃取，三氟乙酸酐（TFAA）衍生化，然后在选择性离子监测（SIM）模式下采用气相色谱-质谱（GS-MS）分析。结果：采用SIM法，加样回收率大于90%。该方法在日内、日间精密度、准确度、线性等方面均可靠。该方法应用于动物实验对象，以测定腹腔（ip）给药后体内[-]- dom脑水平。我们的研究结果表明，[-]- dom的峰值水平与药物刺激控制建立的75分钟预处理时间不一致。讨论：本研究展示了一种灵敏和精确的分析方法，用于测定大鼠大脑中与行为相关剂量的[-]- dom水平。(C) 2001爱思唯尔科学公司版权所有。
Introduction: Indolamine and phenethylamine hallucinogens are drugs of abuse and, as well, mimic some aspects of idiopathic psychosis. To assist in investigating the mechanisms of action of (-)2,5-dimethoxy-4-methylamphetamine ([-]-DOM), a member of the phenethylamine class of serotonergic hallucinogens, a sensitive and precise method for determining its levels in the brain tissue is required. Methods: We now describe a method for determining nanogram quantities of [ -]-DOM in the rat brain tissue using D-amphetamine as an internal standard. The method employs solvent extraction with toluene and derivatization with trifluoroacetic acid anhydride (TFAA) followed by analysis using gas chromatography-mass spectrometry (GS-MS) in the selective ion monitoring (SIM) mode. Results: With SIM detection, our overall recoveries were greater than 90%. The method was reliable in terms of within-day and between-day precision, accuracy, and linearity. The procedure was applied to animal subjects to determine the in vivo [ -]-DOM brain levels following intraperitoneal (ip) administration. Our findings indicate that peak levels of [ -]-DOM do not coincide with the 75-min pretreatment time established by drug-induced stimulus control. Discussion: This study demonstrates a sensitive and precise analytical method for the determination of [ -]-DOM levels in the rat brain following systemic administration of behaviorally relevant doses. (C) 2001 Elsevier Science Inc. All rights reserved.