The characterization fo the B-cell repertoire specific for the 2,4-dinitrophenyl and 2,4,6-trinitrophenyl determinants in neonatal BALB/c mice.
The characterization fo the B-cell repertoire specific for the 2,4-dinitrophenyl and 2,4,6-trinitrophenyl determinants in neonatal BALB/c mice.
复制标题
针对新生儿BALB/C小鼠中的2,4-二硝基苯基和2,4,6-三硝基苯基决定因素的B细胞库的表征。
DOI：
10.1084/jem.141.5.1133
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发表时间：
1975-05-01
影响因子：
15.3
通讯作者：
Press, J L
中科院分区：
文献类型：
--
作者：
Klinman, N R;Press, J L
根据新生儿B细胞刺激的特异性以及新生儿群体中可用的特异性的多样性，分析了BALB/c新生儿中对DNP和TNP半抗原决定簇应答的（B细胞）库。结果表明，新生儿B细胞的刺激参数与非免疫成人的相似，特别是在精细特异性刺激过程中，该过程容易区分半抗原，如2，4-二硝基苯基（DNP）和2，4，6-三硝基苯基（TNP）。用等电聚焦法分析了分离的新生儿BALB/c脾B细胞的抗DNP和抗TNP单克隆抗体的克隆型。在新生儿生命的前4天，几乎所有的抗DNP特异性克隆都是显示pI为5.05、5.25或5.55的IgM抗体的克隆型。这些可以与对TNP应答的克隆型区分开，TNP也主要具有三种不同的pI，5.00、5.15或5.40。这些克隆型代表了生命前4天期间绝大多数DNP和TNP特异性抗体能力，到第6天代表了不到一半的克隆，到第9天是少数。观察到单个1- 4天大的供体具有许多代表给定的主要克隆型的B细胞，这是细胞特异性预定型的证据，并表明预定型B细胞的克隆作为正常的、抗原非依赖性的生殖过程的产物存在。在近交系新生儿中经常出现的克隆型的观察证明了这些克隆型的“生殖系”起源;然而，这些克隆型从供体到供体的发生差异意味着它们的表达中的随机元素。这一发现，几个克隆型反复出现在新生儿发育早期的高数字，而其他克隆型只偶尔发生在早期，已被解释为反映了一个连续的个体发育表达的克隆型。因此，在新生儿中占优势的DNP-和TNP-特异性克隆型可以被看作是5，000 - 10，000个克隆型的代表，这些克隆型早在妊娠第15至17天就表达，而大多数克隆型出现在妊娠第18天之后。
The (B-cell) repertoire responsive to the DNP and TNP haptenic determinants in BALB/c neonates was analyzed in terms of the specificity of stimulation of neonatal B cells as well as the diversity of specificities available in neonatal populations. The results indicate that the parameters of stimulation of neonatal B cells are similar to those of nonimmune adults, particularly in the exquisitely specific stimulatory process which readily discriminates between haptens as closely related as 2,4-dinitrophenyl (DNP) and 2,4,6- trinitrophenyl (TNP). The clonotypes of monoclonal anti-DNP and anti- TNP antibodies derived from isolated neonatal BALB/c splenic B cells in fragment culture were analyzed by isoelectric focusing. During the first 4 days of neonatal life almost all of the anti-DNP-specific clones were of clonotypes displaying IgM antibodies with pI's of 5.05, 5.25, or 5.55. These could be distinguished from clonotypes responding to TNP which were also predominantly of three distinct pI's, 5.00, 5.15 or 5.40. These clonotypes, which represent the vast majority of the DNP- and TNP-specific antibody capability during the first 4 days of life, represented less than half of the clones by day 6 and were a small minority by day 9. The observation that individual 1--4-day-old donors had many B cells representative of a given predominant clonotype is evidence for cellular precommitment of specificity and indicates that clones of precommitted B cells exist as the products of normal, antigen- independent, generative processes. The observation of frequently recurring clonotypes in inbred neonates attests to the "germ line" origin of these clonotypes; however, variance in the occurrence of these clonotypes from donor to donor implies a random element in their expression. The finding that several clonotypes occur repeatedly in high numbers early in neonatal development, while other clonotypes occur only sporadically at early times, has been interpreted as a reflection of a sequential ontogenic expression of clonotypes. Thus the DNP- and TNP-specific clonotypes which predominate in neonates may be seen as representative of a total of 5,000-10,000 clonotypes which are expressed as early as the 15th to 17th day of gestation while most clonotypes appear after the 18th day of gestation.