Gene flow from glufosinate-resistant transgenic hybrid rice Xiang 125S/Bar68-1 to weedy rice and cultivated rice under different experimental designs
Gene flow from glufosinate-resistant transgenic hybrid rice Xiang 125S/Bar68-1 to weedy rice and cultivated rice under different experimental designs
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DOI：
10.1007/s10681-015-1370-y
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发表时间：
2015-02
期刊：
影响因子：
1.9
通讯作者：
G. Sun;Weimin Dai;Rongrong Cui;S. Qiang;Xiaoling Song
中科院分区：
文献类型：
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作者：
G. Sun;Weimin Dai;Rongrong Cui;S. Qiang;Xiaoling Song
从转基因水稻到杂草和栽培水稻的基因流动是一个关键的生物安全考虑。本研究以抗草铵膦（GR）转基因杂交水稻（Oryza sativa L.）到6个杂草稻（O. sativa）和水稻栽培品种（O.采用同心圆、交替栽培和相邻栽培三种试验设计。在同心圆试验中，从GR水稻到7个受体的基因流最大值（0.302%）出现在距花粉供体1 m处，最小值出现在距花粉供体12 m处，为0.011%。对于所有的收件人，基因流频率下降，从花粉源的距离增加。检测到基因流的最大距离为14 m。杂种的分布明显不对称，主要发生在主风向生长的受体植株中。在交替栽培和相邻栽培试验设计中，从GR稻到杂草稻的平均基因流频率分别为0.395 - 0.470%和0- 0.187%。总体而言，花粉供体和受体之间开花重叠的持续时间较长，导致基因流增加。除了表征从GR转基因杂交稻到杂草和栽培稻的基因流之外，本研究还提供了相当多的见解，以选择适当的实验设计来评估基因流，这对于转基因杂交稻的可能部署的风险评估和决策是必要的。
Gene flow from transgenic rice to weedy and cultivated rice is a key biosafety consideration. This study assessed the gene flow from glufosinate-resistant (GR) transgenic hybrid rice (Oryza sativaL.) to six weedy rice (O. sativa) accessions and a rice cultivar (O. sativa) using three experimental designs: concentric circular, alternating cultivation and adjacent cultivation. Maximum gene flow from GR rice to the seven recipients (0.302 %) was found at 1 m from the pollen donor in the concentric circular experiment; the minimum rate was 0.011 % at 12 m from the pollen donor. For all recipients, gene flow frequency declined considerably with increasing distance from the pollen source. The maximum distance at which gene flow was detected was 14 m. A clear asymmetric distribution of hybrids was found with hybridization predominantly occurring in the recipient plants growing in the direction of the prevailing wind. Average gene flow frequency from GR rice to weedy rice ranged from 0.395 to 0.470 % and 0–0.187 % in the alternating and adjacent cultivation experimental designs, respectively. Overall, longer durations in flowering overlap between the pollen donor and the recipients resulted in increased gene flow. In addition to characterizing gene flow from GR transgenic hybrid rice to weedy and cultivated rice, this study provides considerable insight into the selection of a proper experimental design to evaluate gene flow, which is necessary for risk assessment and decision making regarding the possible deployment of transgenic hybrid rice.