Novel lipid mixtures based on synthetic ceramides reproduce the unique stratum corneum lipid organization
Novel lipid mixtures based on synthetic ceramides reproduce the unique stratum corneum lipid organization
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DOI：
10.1194/jlr.m300484-jlr200
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发表时间：
2004-05-01
影响因子：
6.5
通讯作者：
Bouwstra, JA
中科院分区：
文献类型：
--
作者：
de Jager, MW;Gooris, GS;Bouwstra, JA
皮肤最外层的脂质薄片可以保护身体免受失控的水分流失。在人体角质层（SC）中，存在两个结晶层状相，其中主要含有胆固醇、游离脂肪酸和九种游离神经酰胺。先前的研究表明，SC 脂质组织可以用基于分离的 SC 脂质的模型混合物来模拟。然而，这些研究受到天然组织的低可用性和高个体间变异性的阻碍。为了阐明每种脂质类别在形成有效皮肤屏障中的作用，使用合成脂质将提供一种替代方案。本研究的小角和广角X射线衍射结果首次表明，仅含有三种合成神经酰胺的合成脂质混合物在很大程度上反映了SC脂质组织。适当选择的制备方法和脂质成分都会促进两个特征性层状相的形成，其重复距离与天然 SC 中发现的相似。从所有检查的合成脂质混合物中，在 80°C 平衡的胆固醇、神经酰胺和游离脂肪酸的等摩尔混合物在室温和高温下都最大程度地类似于层状和侧向 SC 脂质组织。
Lipid lamellae present in the outermost layer of the skin protect the body from uncontrolled water loss. In human stratum corneum (SC), two crystalline lamellar phases are present, which contain mostly cholesterol, free fatty acids, and nine types of free ceramides. Previous studies have demonstrated that the SC lipid organization can be mimicked with model mixtures based on isolated SC lipids. However, those studies are hampered by low availability and high interindividual variability of the native tissue. To elucidate the role of each lipid class in the formation of a competent skin barrier, the use of synthetic lipids would offer an alternative. The small- and wide-angle X-ray diffraction results of the present study show for the first time that synthetic lipid mixtures, containing only three synthetic ceramides, reflect to a high extent the SC lipid organization. Both an appropriately chosen preparation method and lipid composition promote the formation of two characteristic lamellar phases with repeat distances similar to those found in native SC. From all synthetic lipid mixtures examined, equimolar mixtures of cholesterol, ceramides, and free fatty acids equilibrated at 80degreesC resemble to the highest extent the lamellar and lateral SC lipid organization, both at room and increased temperatures.