Entry of peroxidase into neurons of the central and peripheral nervous systems from extracerebral and cerebral blood
Entry of peroxidase into neurons of the central and peripheral nervous systems from extracerebral and cerebral blood
复制标题
过氧化物酶从脑外血和脑血进入中枢和周围神经系统的神经元
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
M. Brightman
中科院分区：
文献类型：
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作者：
R. Broadwell;M. Brightman
在中枢神经系统内的自主神经节前、感觉和下运动神经元胞体，以及具有投射到脑室周围器官的轴突的细胞体，用辣根过氧化物酶（HRP）逆行标记，所述辣根过氧化物酶（HRP）通过脑血和脑外血递送到它们的轴突末端。在将HRP血管注射到小鼠体内后，血源性过氧化物酶穿过肌肉、神经节和所有室周器官中的可渗透血管，但连合下器官除外，在该器官中未发现泄漏。邻近每个可渗透的脑室周围器官的脑实质迅速被蛋白质淹没。注射后4 - 6小时，这种细胞外HRP反应产物消失，8小时后，特定下丘脑核的胞体周围含有HRP阳性颗粒，表明蛋白质的轴突内逆行转运。这样标记的下丘脑神经元被推测发送轴突到室周器官，如正中隆起或神经垂体，包括大细胞神经分泌视上核和室旁核、副大细胞核、小细胞弓状核和一条延伸到内侧视前区的室周细胞带。标记胞体也邻近终板的血管器和布洛卡对角带核的垂直分支。穹窿下器附近未发现类似标记的细胞体。
Autonomic preganglionic, sensory, and lower motoneuron perikarya within the central nervous system, as well as cell bodies with axons projecting to the circumventricular organs, are retrogradely labeled with horseradish peroxidase (HRP) delivered to their axon terminals by cerebral and extracerebral blood. Subsequent to vascular injection of HRP into mice, blood‐borne peroxidase passes across permeable vessels in muscle, ganglia, and in all circumventricular organs except for the subcommissural organ in which no leak could be discerned. Brain parenchyma adjacent to each of the permeable circumventricular organs quickly becomes inundated with the protein. By four to six hours post‐injection, this extracellular HRP reaction product has disappeared, and by eight hours perikarya of specific hypothalamic nuclei contain HRP‐positive granules indicative of the intra‐axonal retrograde transport of the protein. Hypothalamic neurons so labeled are presumed to send axons to such circumventricular organs as the median eminence or neurohypophysis and include neurons of the magnocellular neurosecretory supraoptic and paraventricular nuclei, the accessory magnocellular nuclei, the parvicellular arcuate nucleus, and a band of periventricular cells extending rostrally into the medial preoptic area. Labeled somata are also adjacent to the organum vasculosum of the lamina terminalis and in the vertical limb of the nucleus of the diagonal band of Broca. No similarly labeled cell bodies were identified near the subfornical organ.