Deciphering endogenous and exogenous regulations of anammox consortia in responding to lincomycin by multiomics: quorum sensing and CRISPR system
Deciphering endogenous and exogenous regulations of anammox consortia in responding to lincomycin by multiomics: quorum sensing and CRISPR system
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DOI：
10.1016/j.watres.2023.120061
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发表时间：
2023-05-17
期刊：
影响因子：
12.8
通讯作者：
Jin，Ren-Cun
中科院分区：
文献类型：
--
作者：
Huang，Dong-Qi;Wu，Qian;Jin，Ren-Cun
抗生素的广泛使用创造了抗生素耐药基因（ARGs）富集的环境，这对人类和动物健康造成了高风险。尽管抗生素在废水处理过程中可以被部分吸附和降解，但对微生物对抗生素胁迫的适应机制的全面理解仍然是迫切的。结合宏基因组学和代谢组学，这项研究揭示了厌氧氨氧化菌聚生体可以通过自发改变对代谢物利用的偏好以及与真核生物（如子囊菌门和担子菌门）建立相互作用来适应林可霉素。具体而言，基于群体感应（QS）的微生物调节和由成簇规则间隔短回文重复序列（CRISPR）系统和全局调节基因介导的ARG转移是主要的适应策略。Western blotting结果证实，Cas9和TrfA是导致ARGs转运途径改变的主要原因。这些发现突出了微生物对抗生素胁迫的潜在适应机制，填补了厌氧氨氧化过程中水平基因转移途径的空白，进一步促进了通过分子和合成生物学技术对ARGs的控制。
The widespread use of antibiotics has created an antibiotic resistance genes (ARGs)-enriched environment, which causes high risks on human and animal health. Although antibiotics can be partially adsorbed and degraded in wastewater treatment processes, striving for a complete understanding of the microbial adaptive mechanism to antibiotic stress remains urgent. Combined with metagenomics and metabolomics, this study revealed that anammox consortia could adapt to lincomycin by spontaneously changing the preference for metabolite utilization and establishing interactions with eukaryotes, such as Ascomycota and Basidiomycota. Specifically, quorum sensing (QS) based microbial regulation and the ARGs transfer mediated by clustered regularly interspaced short palindromic repeats (CRISPR) system and global regulatory genes were the principal adaptive strategies. Western blotting results validated that Cas9 and TrfA were mainly responsible for the alteration of ARGs transfer pathway. These findings highlight the potential adaptative mechanism of microbes to antibiotic stress and fill gaps in horizontal gene transfer pathways in the anammox process, further facilitating the ARGs control through molecular and synthetic biology techniques.