RNA interfering approach for clarifying the PPARγ pathway using lentiviral vector expressing short hairpin RNA
RNA interfering approach for clarifying the PPARγ pathway using lentiviral vector expressing short hairpin RNA
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DOI：
10.1016/s0014-5793(04)00100-0
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发表时间：
2004-02-27
期刊：
影响因子：
3.5
通讯作者：
Mayumi, T
中科院分区：
文献类型：
--
作者：
Katayama, K;Wada, K;Mayumi, T
过氧化物酶体增殖物激活受体γ（PPARγ）在脂肪细胞分化和胰岛素敏感性中发挥核心作用。尽管 PPARgamma 似乎也调节其他细胞类型（例如淋巴细胞）中的多种细胞过程，但详细机制仍不清楚。在这项研究中，我们建立了慢病毒介导的短发夹RNA表达系统，并鉴定了一种有效的短发夹RNA，它可以抑制PPARγ表达，从而显着抑制3T3-L1细胞中前脂肪细胞向脂肪细胞的分化。我们的 PPARgamma 敲除方法将有助于在体内和体外阐明各种细胞类型中的 PPARgamma 通路，并将促进各种疾病治疗应用的开发。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Peroxisome proliferator-activated receptor gamma(PPARgamma) plays a central role in adipocyte differentiation and insulin sensitivity. Although PPARgamma also appears to regulate diverse cellular processes in other cell types such as lymphocytes, the detailed mechanisms remain unclear. In this study, we established a lentivirus-mediated short hairpin RNA expression system and identified a potent short hairpin RNA which suppresses PPARgamma expression, resulting in marked inhibition of preadipocyte-to-adipocyte differentiation in 3T3-L1 cells. Our PPARgamma-knockdown method will serve to clarify the PPARgamma pathway in various cell types in vivo and in vitro, and will facilitate the development of therapeutic applications for a variety of diseases. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.